Oloyede Hussein O B, Adaja Matthew C, Ajiboye Taofeek O, Salawu Musa O
Department of Biochemistry, University of Ilorin, Ilorin 240001, Nigeria.
Antioxidants, Free Radicals, Functional Foods and Toxicology Research Laboratory, Department of Biological Sciences, Al-Hikmah University, Ilorin 230236, Nigeria.
J Integr Med. 2015 Mar;13(2):105-14. doi: 10.1016/S2095-4964(15)60160-1.
Carica papaya is an important fruit with its seeds used in the treatment of ulcer in Nigeria. This study investigated the anti-ulcerogenic and antioxidant activities of aqueous extract of Carica papaya seed against indomethacin-induced peptic ulcer in male rats.
Thirty male rats were separated into 6 groups (A-F) of five rats each. For 14 d before ulcer induction with indomethacin, groups received once daily oral doses of vehicle (distilled water), cimetidine 200 mg/kg body weight (BW), or aqueous extract of C. papaya seed at doses of 100, 150 or 200 mg/kg BW (groups A, B, C, D, E and F, respectively). Twenty-four hours after the last treatment, groups B, C, D, E and F were treated with 100 mg/kg BW of indomethacin to induce ulcer formation.
Carica papaya seed extract significantly (P< 0.05) increased gastric pH and percentage of ulcer inhibition relative to indomethacin-induced ulcer rats. The extract significantly (P< 0.05) decreased gastric acidity, gastric acid output, gastric pepsin secretion, ulcer index and gastric secretion volume relative to group B. These results were similar to that achieved by pretreatment with cimetidine. Specific activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase in the extract-treated groups (D, E and F) were increased significantly over the group B (P< 0.05). Pretreatment with the seed extract protected rats from the indomethacin-mediated decrease in enzyme function experienced by the group B. Similarly, indomethacin-mediated decrease in reduced glutathione level and indomethacin-mediated increase in malondialdehyde were reversed by Carica papaya extract.
In this study, pretreatment with aqueous extract of Carica papaya seed exhibited anti-ulcerogenic and antioxidant effects, which may be due to the enhanced antioxidant enzymes.
番木瓜是一种重要的水果，在尼日利亚其种子被用于治疗溃疡。本研究调查了番木瓜种子水提取物对吲哚美辛诱导的雄性大鼠消化性溃疡的抗溃疡和抗氧化活性。
将30只雄性大鼠分成6组（A - F），每组5只。在用吲哚美辛诱导溃疡前14天，各组大鼠每天口服一次赋形剂（蒸馏水）、200 mg/kg体重的西咪替丁或100、150或200 mg/kg体重的番木瓜种子水提取物（分别为A、B、C、D、E和F组）。最后一次治疗24小时后，B、C、D、E和F组用100 mg/kg体重的吲哚美辛处理以诱导溃疡形成。
相对于吲哚美辛诱导的溃疡大鼠，番木瓜种子提取物显著（P < 0.05）提高了胃pH值和溃疡抑制百分比。相对于B组，该提取物显著（P < 0.05）降低了胃酸度、胃酸分泌量、胃蛋白酶分泌、溃疡指数和胃分泌量。这些结果与西咪替丁预处理所取得的结果相似。提取物处理组（D、E和F）中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和葡萄糖 - 6 - 磷酸脱氢酶的比活性相对于B组显著增加（P < 0.05）。种子提取物预处理可保护大鼠免受B组所经历的吲哚美辛介导的酶功能下降的影响。同样，番木瓜提取物可逆转吲哚美辛介导的还原型谷胱甘肽水平降低和吲哚美辛介导的丙二醛增加。
在本研究中，番木瓜种子水提取物预处理表现出抗溃疡和抗氧化作用，这可能归因于抗氧化酶的增强。
