Lee Jung Woo, Han Yea Sik, Kim Sin Rak, Kim Han Kyeol, Kim Hyun, Park Jin Hyung
Department of Plastic and Reconstructive Surgery, Kosin University College of Medicine, Busan, Korea.
Department of Anatomy, Kosin University College of Medicine, Busan, Korea.
Arch Plast Surg. 2015 Mar;42(2):150-8. doi: 10.5999/aps.2015.42.2.150. Epub 2015 Mar 16.
Fat is widely used in soft tissue augmentation. Nevertheless, it has an unpredictably high resorption rate. Clinically, external expansion with negative pressure is used to increase fat graft survival. In this study, fat graft recipient sites were preconditioned by external application of negative pressure in order to test for improvements in vascularity and fat graft survival.
Negative pressure was applied randomly to either the left or right dorsal ear of 20 New Zealand male white rabbits at a pressure of -125 mm Hg. The negative pressure was removed one week after the skin perfusion was measured. The skin flap at each ear was elevated, and 1 g of fat was grafted above the dorsal perichondrium. After one week, the fat weight, microvessel density, mature vessel density of the skin and fat, and amount of glycerol released were measured. Three months after the grafting, the same measurements were performed, with the exception of glycerol release.
The fat survival rate of the experimental group (75.4%±3.9%) was higher than that of the control group (53.1%±4.3%) (P<0.001). Skin perfusion was higher in the experimental group. The glycerol release in the experimental group was significantly higher than in the control. The microvessel density of the skin and fat was significantly higher in the experimental group. Three months after the grafting, the skin and fat mature vessel density was significantly higher in the experimental groups.
Negative pressure prior to fat grafting increased the vascularity of the recipient site, and, accordingly, enhanced fat graft survival.
脂肪广泛应用于软组织填充。然而，其吸收率高得难以预测。临床上，负压外部扩张用于提高脂肪移植的存活率。在本研究中，通过外部施加负压对脂肪移植受区进行预处理，以测试血管生成和脂肪移植存活率的改善情况。
对20只新西兰雄性白兔的左耳或右耳随机施加-125 mmHg的负压。在测量皮肤灌注一周后去除负压。掀起每只耳朵的皮瓣，在软骨膜上方移植1 g脂肪。一周后，测量脂肪重量、皮肤和脂肪的微血管密度、成熟血管密度以及甘油释放量。移植三个月后，除甘油释放量外，进行相同的测量。
实验组的脂肪存活率（75.4%±3.9%）高于对照组（53.1%±4.3%）（P<0.001）。实验组的皮肤灌注较高。实验组的甘油释放量显著高于对照组。实验组皮肤和脂肪的微血管密度显著更高。移植三个月后，实验组的皮肤和脂肪成熟血管密度显著更高。
脂肪移植前的负压增加了受区的血管生成，因此提高了脂肪移植的存活率。
