Department of Dermatology, Spedali civili di Brescia, Brescia, Italy.
Department of Clinical and Experimental Sciences, Pediatrics Clinic and Institute for Molecular Medicine A. Nocivelli, University of Brescia, Spedali Civili di Brescia, Brescia, Italy.
Int Wound J. 2016 Dec;13(6):1104-1110. doi: 10.1111/iwj.12415. Epub 2015 Mar 19.
Senescent fibroblasts, which are present in chronic ulcers, are the reason for the wound becoming chronic. In this study, we introduce full-thickness micro skin grafts in the ulcer, a surgical technique known as a 'nested graft', which gave encouraging results leading to complete wound healing in all patients. The assessment of fibroblast cultures taken from the wound before and after treatment and comparison with fibroblasts from healthy skin showed that the fibroblasts taken from the ulcer after the nested graft treatment acquire morpho-functional characteristics overlapping those of fibroblasts from healthy skin. This surgical approach is, therefore, able to lead to the healing of chronic ulcers through the de-senescence of the fibroblasts.
存在于慢性溃疡中的衰老成纤维细胞是导致伤口变成慢性的原因。在这项研究中，我们在溃疡中引入了全厚微皮移植，这是一种被称为“嵌套移植”的手术技术，结果令人鼓舞，所有患者的伤口都完全愈合。对治疗前后从伤口中取出的成纤维细胞进行评估，并与健康皮肤的成纤维细胞进行比较，结果表明，从嵌套移植治疗后的溃疡中取出的成纤维细胞获得了与健康皮肤成纤维细胞重叠的形态功能特征。因此，这种手术方法能够通过成纤维细胞去衰老来促进慢性溃疡的愈合。
