Meléndez-Lazo A, Tvarijonaviciute A, Cerón J J, Planellas M, Pastor J
Department de Medicina I Cirugía Animals, Universitat Autónoma de Barcelona, Bellaterra, Barcelona, Spain; Hospital Clínic Veterinari, Universitat Autónoma de Barcelona, Bellaterra, Barcelona, Spain.
Department de Medicina I Cirugía Animals, Universitat Autónoma de Barcelona, Bellaterra, Barcelona, Spain; Department of Animal Medicine and Surgery, Veterinary School, University of Murcia, Murcia, Spain.
J Comp Pathol. 2015 May;152(4):304-12. doi: 10.1016/j.jcpa.2015.01.008. Epub 2015 Mar 21.
Anaemia secondary to inflammatory disease is one of the main causes of anaemia in veterinary and human medicine and impairment of iron homeostasis due to the release of pro-inflammatory cytokines is one of the aetiological mechanisms involved. Because reticulocytes are recently produced cells, reticulocyte indices are early indicators of iron deficiency anaemia in man and dogs and reticulocyte indices may be affected during the course of inflammatory processes earlier than indices related to mature red blood cells. The aim of this study was to evaluate the possible influence of inflammation on reticulocyte parameters including concentration, mean reticulocyte volume, volume distribution width, percentage of microcytic reticulocytes, percentage of macrocytic reticulocytes, mean reticulocyte haemoglobin content (CHr), haemoglobin distribution width, cell haemoglobin concentration, mean percentage of hypochromic reticulocytes, percentage of reticulocytes with low CHr and immature reticulocyte factor medium and high, and on white blood cell concentration by using C-reactive protein (CRP) as an inflammatory biomarker. Samples from 175 diseased dogs and 16 healthy dogs were included in the study. The diseased dogs were grouped according to plasma CRP and ferritin concentrations, the presence and type of anaemia and different aetiopathological categories. Dogs with high plasma CRP concentrations had lower CHr (median 23.3 pg) and percentage of reticulocytes with high CHr (median 35.5%) and higher percentage of reticulocytes with low CHr (median 14.6%) compared with dogs without inflammation (median 24.9 pg, median 50.9% and median 7.8%, respectively) and healthy dogs (median 25.1 pg, median 50.0% and median 6.1%, respectively), with no differences between the last two groups. Reticulocyte parameters, particularly those related to haemoglobin concentration, are therefore affected by inflammatory conditions in anaemic and in non-anaemic dogs.
炎症性疾病继发的贫血是兽医和人类医学中贫血的主要原因之一，促炎细胞因子释放导致铁稳态受损是其中的病因机制之一。由于网织红细胞是最近生成的细胞，网织红细胞指数是人及犬缺铁性贫血的早期指标，且在炎症过程中，网织红细胞指数可能比与成熟红细胞相关的指标更早受到影响。本研究的目的是通过使用C反应蛋白（CRP）作为炎症生物标志物，评估炎症对网织红细胞参数（包括浓度、平均网织红细胞体积、体积分布宽度、小细胞网织红细胞百分比、大细胞网织红细胞百分比、平均网织红细胞血红蛋白含量（CHr）、血红蛋白分布宽度、细胞血红蛋白浓度、低色素网织红细胞平均百分比、CHr低的网织红细胞百分比以及未成熟网织红细胞因子中、高）以及白细胞浓度的可能影响。本研究纳入了175只患病犬和16只健康犬的样本。患病犬根据血浆CRP和铁蛋白浓度、贫血的存在及类型以及不同的病因病理类别进行分组。与无炎症的犬（中位数分别为24.9 pg、50.9%和7.8%）和健康犬（中位数分别为25.1 pg、50.0%和6.1%）相比，血浆CRP浓度高的犬CHr较低（中位数为23.3 pg），CHr高的网织红细胞百分比也较低（中位数为35.5%），而CHr低的网织红细胞百分比更高（中位数为14.6%），后两组之间无差异。因此，网织红细胞参数，尤其是与血红蛋白浓度相关的参数，在贫血和非贫血犬中均受炎症状态的影响。
