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利用万带兰（Justicia adhatoda L.）叶提取物合成的银纳米粒子的抗菌活性。

Antibacterial Activity of Green Synthesized Silver Nanoparticles Using Vasaka (Justicia adhatoda L.) Leaf Extract.

机构信息

Department of Botany, Kabi Nazrul College, Murarai, Birbhum, 731219 WB India.

Department of Chemistry, Kabi Nazrul College, Murarai, Birbhum, 731219 WB India.

出版信息

Indian J Microbiol. 2015 Jun;55(2):163-7. doi: 10.1007/s12088-015-0512-1. Epub 2015 Jan 21.

DOI:10.1007/s12088-015-0512-1
PMID:25805902
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4363258/
Abstract

There is an increasing demand for silver nanoparticles due to its wide applicability in various area of biological science such as in field of antimicrobial and therapeutics, biosensing, drug delivery etc. To use in bioprocess the silver nanoparticles should be biocompatible and free from toxic chemicals. In the present study we report a cost effective and environment friendly route for green synthesis of silver nanoparticles using Vasaka (Justicia adhatoda L.) leaf extract as reducing as well as capping agent. This plant has been opted for the present study for its known medicinal properties and it is easily available. The biosynthesized silver nanoparticles are characterized by UV-Vis spectroscopy and TEM analysis. It is observed the nanoparticles are well shaped and the average particle size is 20 nm in the range of 5-50 nm. The antibacterial activity of these nanoparticles against Pseudomonas aeruginosa MTCC 741 has been measured by disc diffusion method, agar cup assay and serial dilution turbidity measurement assay. The results show green synthesized silver nanoparticles, using Vasaka leaf extract, have a potential to inhibit the growth of bacteria.

摘要

由于银纳米粒子在抗菌和治疗、生物传感、药物输送等各个生物科学领域的广泛适用性，对其的需求日益增长。为了在生物工艺中使用，银纳米粒子应该具有生物相容性并且不含有毒化学物质。在本研究中，我们报告了一种使用 Vasaka（Justicia adhatoda L.）叶提取物作为还原剂和稳定剂的绿色合成银纳米粒子的经济有效且环保的方法。选择这种植物进行本研究是因为它具有已知的药用特性，并且易于获得。通过紫外-可见光谱和 TEM 分析对生物合成的银纳米粒子进行了表征。观察到纳米粒子形状良好，平均粒径在 5-50nm 范围内为 20nm。通过圆盘扩散法、琼脂杯法和连续稀释浊度测量法测定了这些纳米粒子对铜绿假单胞菌 MTCC 741 的抗菌活性。结果表明，使用 Vasaka 叶提取物合成的银纳米粒子具有抑制细菌生长的潜力。