Department of Medical Sciences, Dermatology Section, Turin, Italy.
Department of Public Health and Pediatrics, Microbiology Section, Turin, Italy.
J Eur Acad Dermatol Venereol. 2016 Apr;30(4):624-7. doi: 10.1111/jdv.13094. Epub 2015 Mar 23.
The discovery, from 2007, of eight new human polyomaviruses (HPyVs) has revived interest in the Polyomaviridae family and their association with human diseases and cancer. In particular, HPyV6 and HPyV7 were discovered in skin swabs of healthy donors and TSPyV was discovered in a heart transplant recipient affected by virus-associated Trichodysplasia Spinulosa (TS), a rare skin disease, exclusively found in immunocompromised patients.
The presence of HPyV6, HPyV7 and TSPyV DNA in skin biopsies from patients affected by different skin diseases (cancers and inflammatory disorders) has been evaluated to confirm their skin tropism and the possible pathological association.
DNA extracted was amplified with HPyV6, HPyV7 and TSPyV specific PCR real time on Taqman platform with standard profile.
HPyV7 and TSPyV sequences were not found in any skin specimen analysed. HPyV6, on the other hand, was detected in 30% of samples from healthy subjects vs. 14.3% of skin cancer patients and 2.9% of inflammatory disorders. HPyV6 sequences have been detected in primary cutaneous T-cell lymphoma (CTCL) patients (in 18.6% out of Mycosis Fungoides (MF) patients and in 16.7% out of CTCL not MF/SS(Sèzary syndrome) but have not been detected in primary cutaneous B-cell lymphoma (CBCL) patients.
Our preliminary data suggest that these three novel human polyomaviruses seem not to play a significant role neither in the pathogenesis of cutaneous malignancies nor in that of inflammatory disorders but, according to literature, can inhabit the skin. On the basis of our data regarding the HPyV6 DNA presence with decreasing percentages in healthy subjects, skin cancer and inflammatory disorders patients, it could be an intriguing matter to study if the activated innate immune response in inflammatory disorders can suppress the virus. Further investigations are needed to better understand their relationship with the human host and its innate immune system.
自 2007 年以来，八种新的人类多瘤病毒（HPyV）的发现重新引起了人们对多瘤病毒科及其与人类疾病和癌症的关联的兴趣。特别是，在健康供体的皮肤拭子中发现了 HPyV6 和 HPyV7，在一名患有病毒相关的毛发性角化病（TS）的心脏移植受者中发现了 TSPyV，TS 是一种罕见的皮肤病，仅在免疫功能低下的患者中发现。
评估 HPyV6、HPyV7 和 TSPyV DNA 在受不同皮肤疾病（癌症和炎症性疾病）影响的患者皮肤活检中的存在，以确认其皮肤嗜性和可能的病理关联。
使用 Taqman 平台上的 HPyV6、HPyV7 和 TSPyV 特异性 PCR 实时扩增提取的 DNA，采用标准程序。
在分析的任何皮肤标本中均未发现 HPyV7 和 TSPyV 序列。另一方面，在 30%的健康受试者样本中检测到 HPyV6，而在 14.3%的皮肤癌患者和 2.9%的炎症性疾病患者中检测到 HPyV6。在原发性皮肤 T 细胞淋巴瘤（CTCL）患者（蕈样真菌病（MF）患者中 18.6%，非 MF/SS（Sézary 综合征）的 CTCL 患者中 16.7%）中检测到 HPyV6 序列，但在原发性皮肤 B 细胞淋巴瘤（CBCL）患者中未检测到。
我们的初步数据表明，这三种新型人类多瘤病毒似乎在皮肤恶性肿瘤和炎症性疾病的发病机制中都没有发挥重要作用，但根据文献，它们可以栖息在皮肤上。基于我们关于 HPyV6 DNA 在健康受试者、皮肤癌和炎症性疾病患者中以递减百分比存在的资料，可以研究炎症性疾病中激活的先天免疫反应是否可以抑制病毒，这是一个有趣的问题。需要进一步的研究来更好地了解它们与人类宿主及其先天免疫系统的关系。
