Edlich-Muth Christian, Artero Jean-Baptiste, Callow Phil, Przewloka Marcin R, Watson Aleksandra A, Zhang Wei, Glover David M, Debski Janusz, Dadlez Michal, Round Adam R, Forsyth V Trevor, Laue Ernest D
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, CB2 1GA Cambridge, United Kingdom.
Life Sciences Group, Institut Laue-Langevin, 71 Avenue des Martyrs, CS 20156, Grenoble, Cedex 9, France; Faculty of Natural Sciences, Keele University, ST5 5BG Staffordshire, United Kingdom.
J Mol Biol. 2015 May 22;427(10):1949-63. doi: 10.1016/j.jmb.2015.03.010. Epub 2015 Mar 24.
Nucleoplasmin is a histone chaperone that consists of a pentameric N-terminal domain and an unstructured C-terminal tail. The pentameric core domain, a doughnut-like structure with a central pore, is only found in the nucleoplasmin family. Here, we report the first structure of a nucleoplasmin-like domain (NPL) from the unrelated Drosophila protein, FKBP39, and we present evidence that this protein associates with chromatin. Furthermore, we show that two other chromatin proteins, Arabidopsis thaliana histone deacetylase type 2 (HD2) and Saccharomyces cerevisiae Fpr4, share the NPL fold and form pentamers, or a dimer of pentamers in the case of HD2. Thus, we propose a new family of proteins that share the pentameric nucleoplasmin-like NPL domain and are found in protists, fungi, plants and animals.
核质蛋白是一种组蛋白伴侣，由一个五聚体的N端结构域和一个无结构的C端尾巴组成。五聚体核心结构域是一种具有中央孔的甜甜圈状结构，仅在核质蛋白家族中发现。在这里，我们报道了来自不相关的果蝇蛋白FKBP39的核质蛋白样结构域（NPL）的首个结构，并提供证据表明该蛋白与染色质相关。此外，我们表明另外两种染色质蛋白，拟南芥2型组蛋白去乙酰化酶（HD2）和酿酒酵母Fpr4，具有NPL折叠并形成五聚体，或者在HD2的情况下形成五聚体二聚体。因此，我们提出了一个新的蛋白家族，它们共享五聚体核质蛋白样NPL结构域，并且存在于原生生物、真菌、植物和动物中。
