Paris J L, Román J, Manzano M, Cabañas M V, Vallet-Regí M
Departamento de Química Inorgánica y Bioinorgánica, Facultad de Farmacia, UCM, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, 28040 Madrid, Spain; Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.
Departamento de Química Inorgánica y Bioinorgánica, Facultad de Farmacia, UCM, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, 28040 Madrid, Spain.
Int J Pharm. 2015;486(1-2):30-7. doi: 10.1016/j.ijpharm.2015.03.048. Epub 2015 Mar 23.
This work presents the tuning of drug-loaded scaffolds for bone regeneration as dual-drug delivery systems. Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (anti-inflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite. A high-performance liquid chromatography method was optimized to separate and simultaneously quantify the two drugs released from the dual-drug codelivery system. The multifunctional porous scaffolds fabricated show a very rapid delivery of anti-inflammatory (interesting to reduce inflammation after implantation), whereas the anti-osteoporotic drug showed sustained release behaviour (important to promote bone regeneration). Since ibuprofen release was faster than desired, this drug was encapsulated in chitosan spheres which were then incorporated into the scaffolds, obtaining a release profile suitable for clinical application. The results obtained open the possibility to simultaneously incorporate two or more drugs to an osseous implant in a controlled way improving it for bone healing application.
这项工作展示了作为双药递送系统的用于骨再生的载药支架的调控。两种治疗物质，唑来膦酸（抗骨质疏松药物）和布洛芬（抗炎药物）以可控方式成功地掺入三维设计的磷灰石/琼脂糖复合材料多孔支架中。优化了一种高效液相色谱方法，以分离并同时定量从双药共递送系统释放的两种药物。所制备的多功能多孔支架显示出抗炎药物的快速释放（对减少植入后的炎症很重要），而抗骨质疏松药物则表现出持续释放行为（对促进骨再生很重要）。由于布洛芬的释放速度比预期快，因此将该药物封装在壳聚糖球中，然后将其掺入支架中，从而获得适合临床应用的释放曲线。所获得的结果为以可控方式将两种或更多种药物同时掺入骨植入物中以改善其在骨愈合应用中的性能开辟了可能性。
