Laboratory for Cognitive Brain Mapping, RIKEN Brain Science Institute Saitama, Japan ; Graduate School for Science and Engineering, Saitama University Saitama, Japan.
Laboratory for Cognitive Brain Mapping, RIKEN Brain Science Institute Saitama, Japan ; Ichinohe Neural System Group, Laboratory for Molecular Analysis of Higher Cognitive Function, RIKEN Brain Science Institute Saitama, Japan.
Front Neuroanat. 2015 Mar 12;9:30. doi: 10.3389/fnana.2015.00030. eCollection 2015.
Neuronal activities recorded from the dorsal bank of the anterior cingulate sulcus have suggested that this cortical area is involved in control of search vs. repetition, goal-based action selection and encoding of prediction error regarding action value. In this study, to explore potential anatomical bases for these neuronal activities, we injected retrograde tracers (CTB-Alexa-488 and CTB-gold) into the dorsal bank of the anterior cingulate sulcus and examined the distribution of labeled cell bodies in macaque monkey brains. The Nissl staining showed that the cortex in the dorsal bank of the anterior cingulate sulcus has consistent layer 4 which means that the cortical region is a part of the granular prefrontal cortex. The injection site belonged to the sulcal portion of area 9m in two cases and the sulcal portion of area 8Bm in one case. In addition to the continuous distribution of labeled cells in the two areas (areas 9m and 8Bm) around the injection site, the labeled cells were densely distributed in the cingulate areas (areas 24, 32, and 23) in all the cases. The dense labeling of cells was also found in other prefrontal areas (areas 46, 10, 11, and 12) in the two cases with injection into the sulcal portion of area 9m, whereas the dense labeling of cells was found in pre-motor areas (F6 and F7) in the case with injection into the sulcal portion of area 8Bm. The dense labeling of cells in the prefrontal and premotor areas was more similar to those previously found after injections into dorsal parts of areas 9 and 8B. Subcortical distribution of labeled cells was found in the mediodorsal nucleus of thalamus, claustrum, and substantia nigra pars compacta in all the cases.
从前扣带回皮质背侧Bank 记录的神经元活动表明，该皮质区域参与控制搜索与重复、基于目标的动作选择以及动作值的预测误差编码。在这项研究中，为了探索这些神经元活动的潜在解剖学基础，我们将逆行示踪剂（CTB-Alexa-488 和 CTB-金）注射到前扣带回皮质背侧Bank，并检查了猕猴大脑中标记细胞体的分布。尼氏染色显示，前扣带回皮质背侧Bank 的皮质具有一致的第 4 层，这意味着该皮质区域是颗粒前额皮质的一部分。注射部位在两个病例中属于 9m 区的沟部，在一个病例中属于 8Bm 区的沟部。除了在注射部位周围的两个区域（9m 区和 8Bm 区）中连续分布的标记细胞外，在所有病例中，标记细胞还密集分布在扣带区域（24、32 和 23 区）。在两个注射到 9m 区沟部的病例中，还发现了其他前额皮质区域（46、10、11 和 12 区）的细胞密集标记，而在一个注射到 8Bm 区沟部的病例中，发现了运动前区域（F6 和 F7）的细胞密集标记。前额皮质和运动前区域的细胞密集标记与之前在 9 和 8B 的背侧部分注射后发现的标记更为相似。在所有病例中，标记细胞的皮质下分布还在前脑岛核、屏状核和黑质致密部被发现。
