Li Ningyue, Xu Yanhua, Chen Xiaojuan, Duan Qing, Zhao Mei
Key Lab of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China.
Int J Mol Sci. 2015 Mar 25;16(4):6831-41. doi: 10.3390/ijms16046831.
The link between alterations in circadian rhythms and depression are well established, but the underlying mechanisms are far less elucidated. We investigated the circadian characteristics of immobility behavior in wild type (WT) mice and mice with mutations in core Clock genes.
All mice were tested with forced swim test (FST) at 4 h intervals.
These experiments revealed significant diurnal rhythms associated with immobility behavior in both male and female WT mice with sex-different circadian properties. In addition, male mice showed significantly less immobility during the night phase in comparison to female mice. Female Per1Brdm1 mice also showed significant rhythmicity. However, the timing of rhythmicity was very different from that observed in female wild type mice. Male Per1Brdm1 mice showed a pattern of rhythmicity similar to that of wild type mice. Furthermore, female Per1Brdm1 mice showed higher duration of immobility in comparison to male Per1Brdm1 mice in both daytime and early night phases. Neither Per2Brdm1 nor ClockΔ19 mice showed significant rhythmicity, but both female Per2Brdm1 and ClockΔ19 mice had lower levels of immobility, compared to males.
This study highlights the differences in the circadian characteristics of immobility induced by FST in WT, ClockΔ19, Per1, and Per2 deficient mice.
昼夜节律改变与抑郁症之间的联系已得到充分证实，但其潜在机制仍有待深入阐明。我们研究了野生型（WT）小鼠和核心生物钟基因发生突变的小鼠静止行为的昼夜节律特征。
所有小鼠每隔4小时进行一次强迫游泳试验（FST）。
这些实验揭示了雄性和雌性WT小鼠中与静止行为相关的显著昼夜节律，且具有性别差异的昼夜特性。此外，与雌性小鼠相比，雄性小鼠在夜间的静止时间明显更少。雌性Per1Brdm1小鼠也表现出显著的节律性。然而，其节律时间与雌性野生型小鼠观察到的情况非常不同。雄性Per1Brdm1小鼠表现出与野生型小鼠相似的节律模式。此外，在白天和傍晚早期，雌性Per1Brdm1小鼠的静止时间比雄性Per1Brdm1小鼠更长。Per2Brdm1小鼠和ClockΔ19小鼠均未表现出显著的节律性，但与雄性相比，雌性Per2Brdm1小鼠和ClockΔ19小鼠的静止水平均较低。
本研究突出了野生型、ClockΔ19、Per1和Per2基因缺陷小鼠中FST诱导的静止行为昼夜节律特征的差异。
