Mizuki K, Nose K, Okamoto H, Tsuchida N, Hayashi K
Biochem Biophys Res Commun. 1985 Apr 30;128(2):1037-43. doi: 10.1016/0006-291x(85)90152-4.
The expression of c-oncogenes was examined with normal human fibroblasts (WI-38) and the cells transformed in vitro by gamma-irradiation (CT-1). The amount of Ki-ras-specific mRNA in CT-1 cells was found to be approximately twice that in WI-38 cells. By Southern blot hybridization, the c-Ki-ras 2 gene of CT-1 cells was found to be amplified about two-fold. CT-1 cells have higher levels of c-myc mRNA than normal cells, although the gene dosage and the restriction nuclease pattern of the c-myc gene was the same. The content of c-myc mRNA in CT-1 cells was found to be constitutively high, whereas that in normal cells was increased by serum addition.
用正常人成纤维细胞（WI-38）和经γ射线体外转化的细胞（CT-1）检测了原癌基因的表达。发现CT-1细胞中Ki-ras特异性mRNA的量约为WI-38细胞中的两倍。通过Southern印迹杂交，发现CT-1细胞的c-Ki-ras 2基因扩增了约两倍。CT-1细胞的c-myc mRNA水平高于正常细胞，尽管c-myc基因的基因剂量和限制性核酸酶图谱相同。发现CT-1细胞中c-myc mRNA的含量持续较高，而正常细胞中的c-myc mRNA含量则因添加血清而增加。
