McLaughlin Heather P, Motherway Mary O'Connell, Lakshminarayanan Bhuvaneswari, Stanton Catherine, Paul Ross R, Brulc Jennifer, Menon Ravi, O'Toole Paul W, van Sinderen Douwe
School of Microbiology, University College Cork, Cork, Ireland; Alimentary Pharmabiotic Centre, Cork, Ireland.
Teagasc Moorepark, Fermoy, Co. Cork, Ireland; Alimentary Pharmabiotic Centre, Cork, Ireland.
Int J Food Microbiol. 2015 Jun 16;203:109-21. doi: 10.1016/j.ijfoodmicro.2015.03.008. Epub 2015 Mar 10.
Because increased proportions of particular commensal bacteria such as bifidobacteria and lactobacilli have been linked to human health through a variety of mechanisms, there is corresponding interest in identifying carbohydrates that promote growth and metabolic activity of these bacteria. We evaluated the ability of 20 carbohydrates, including several commercially available carbohydrates that are sold as prebiotic ingredients, to support growth of 32 human-derived isolates belonging to the genera Bifidobacterium and Lactobacillus, including those isolated from healthy elderly subjects. In general, bifidobacterial strains were shown to display more diverse carbohydrate utilization profiles compared to the tested Lactobacillus species, with several bifidobacterial strains capable of metabolizing xylo-oligosaccharide (XOS), arabinoxylan, maltodextrin, galactan and carbohydrates containing fructo-oligosaccharide (FOS) components. In contrast, maltodextrin, galactan, arabinogalactan and galactomannan did not support robust growth (≥0.8 OD600 nm) of any of the Lactobacillus strains assessed. Carbohydrate fermentation was variable among strains tested of the same species for both genera. This study advances our knowledge of polysaccharide utilization by human gut commensals, and provides information for the rational design of selective prebiotic food ingredients.
由于特定共生细菌（如双歧杆菌和乳酸杆菌）比例的增加已通过多种机制与人类健康相关联，因此人们对识别促进这些细菌生长和代谢活性的碳水化合物产生了相应的兴趣。我们评估了20种碳水化合物（包括几种作为益生元成分出售的市售碳水化合物）支持32株源自人类的双歧杆菌属和乳酸杆菌属菌株生长的能力，这些菌株包括从健康老年受试者中分离出的菌株。总体而言，与测试的乳酸杆菌种类相比，双歧杆菌菌株显示出更多样化的碳水化合物利用谱，有几种双歧杆菌菌株能够代谢木寡糖（XOS）、阿拉伯木聚糖、麦芽糊精、半乳聚糖和含有低聚果糖（FOS）成分的碳水化合物。相比之下，麦芽糊精、半乳聚糖、阿拉伯半乳聚糖和半乳甘露聚糖不支持所评估的任何乳酸杆菌菌株的强劲生长（≥0.8 OD600 nm）。两个属中同一物种的测试菌株之间的碳水化合物发酵情况各不相同。这项研究增进了我们对人类肠道共生菌利用多糖的了解，并为合理设计选择性益生元食品成分提供了信息。
