Kellawan J Mikhail, Johansson Rebecca E, Harrell John W, Sebranek Joshua J, Walker Benjamin J, Eldridge Marlowe W, Schrage William G
Bruno Balke Biodynamics Lab, Department of Kinesiology, School of Education, University of Wisconsin-Madison, 1141A Natatorium, 2000 Observatory Drive, Madison, WI, 53706, USA,
Eur J Appl Physiol. 2015 Aug;115(8):1735-46. doi: 10.1007/s00421-015-3160-6. Epub 2015 Mar 28.
We hypothesized exercise vasodilation would be greater in women due to nitric oxide synthase (NOS) and cyclooxygenase (COX) signaling.
45 healthy adults (23 women, W, 22 men, M, 26 ± 1 years) completed two 10-min trials of dynamic forearm exercise at 15 % intensity. Forearm blood flow (FBF; Doppler ultrasound), arterial pressure (brachial catheter), and forearm lean mass were measured to calculate relative forearm vascular conductance (FVCrel) = FBF 100 mmHg(-1) 100 g(-1) lean mass. Local intra-arterial infusion of L-NMMA or ketorolac acutely inhibited NOS and COX, respectively. In Trial 1, the first 5 min served as control exercise (CON), followed by 5 min of L-NMMA or ketorolac over the last 5 min of exercise. In Trial 2, the remaining drug was infused during 5-10 min, to achieve combined NOS-COX inhibition (double blockade, DB).
Are mean ± SE. Women exhibited 29 % greater vasodilation in CON (ΔFVCrel, 19 ± 1 vs. 15 ± 1, p = 0.01). L-NMMA reduced ΔFVCrel (p < 0.001) (W: Δ -2.3 ± 1.3 vs. M: Δ -3.7 ± 0.8, p = 0.25); whereas, ketorolac modestly increased ΔFVCrel (p = 0.04) similarly between sexes (W: Δ 1.6 ± 1.1 vs. M: Δ 2.0 ± 1.6, p = 0.78). DB was also found to be similar between the sexes (p = 0.85).
These data clearly indicate women produce a greater exercise vasodilator response. Furthermore, contrary to experiments in animal models, these data are the first to demonstrate vascular control by NOS and COX is similar between sexes.
我们假设由于一氧化氮合酶（NOS）和环氧化酶（COX）信号传导，女性的运动性血管舒张作用会更强。
45名健康成年人（23名女性，W；22名男性，M；年龄26±1岁）以15%的强度完成了两次10分钟的动态前臂运动试验。测量前臂血流量（FBF；多普勒超声）、动脉压（肱动脉导管）和前臂去脂体重，以计算相对前臂血管传导率（FVCrel）=FBF×100 mmHg⁻¹×100 g⁻¹去脂体重。局部动脉内输注L-NMMA或酮咯酸分别急性抑制NOS和COX。在试验1中，前5分钟作为对照运动（CON），在运动的最后5分钟内接下来的5分钟输注L-NMMA或酮咯酸。在试验2中，在5-10分钟内输注剩余药物，以实现NOS-COX联合抑制（双重阻断，DB）。
数据为均值±标准误。女性在CON中的血管舒张作用强29%（ΔFVCrel，19±1对15±1，p = 0.01）。L-NMMA降低了ΔFVCrel（p < 0.001）（女性：Δ -2.3±1.3对男性：Δ -3.7±0.8，p = 0.25）；而酮咯酸适度增加了ΔFVCrel（p = 0.04），两性间相似（女性：Δ 1.6±1.1对男性：Δ 2.0±1.6，p = 0.78）。还发现两性间DB相似（p = 0.85）。
这些数据清楚地表明女性产生更强的运动性血管舒张反应。此外，与动物模型实验相反，这些数据首次证明NOS和COX对血管的控制在两性间相似。
