Dept. of Biology, University of Rome "Tor Vergata", Via della Ricerca Scientifica 1, 00133, Rome, Italy.
Food Funct. 2015 May;6(5):1526-38. doi: 10.1039/c5fo00110b.
Vitis vinifera L. is a plant species rich in phenolic compounds that are usually associated with the health benefits of wine and grape consumption in the diet. Anthocyanins, catechins, flavonol, phenolic acids and stilbenes are key molecular constituents of the Vitis berries, affecting the quality of grape products. The purpose of this work was to identify the metabolic profiles of 37 genetically certified V. vinifera Latial accessions. In particular, qualitative and quantitative analyses of specific secondary metabolites and total phenolic and tannin contents were performed by LC-MS and spectrophotometric analysis. In addition, since plant molecules are well-known for their free radical scavenging properties, the antioxidant effects of the sample extracts were evaluated through two different antiradical assays: DPPH and FRAP tests. Finally, a preliminary screening of the antiproliferative activity of each specimen on HCT-116 human colorectal cancer cells was conducted. All the results showed a great variety and amount of phenolic compounds in all accessions; moreover, we observed a significant correlation in the extracts between the metabolite concentration and bioactivity. Besides, some samples presented extraordinary biological effects, such as reduction of tumor cell growth not associated with cytotoxicity, supporting their use as possible future adjuvants for cancer therapy. In conclusion, the present research increased the scientific knowledge about Italian autochthonous vine ecotypes in order to valorize them and support their reintroduction in the local economic system.
葡萄(Vitis vinifera L.)是一种富含酚类化合物的植物物种，通常与葡萄酒和葡萄在饮食中的健康益处有关。花色苷、儿茶素、类黄酮、酚酸和白藜芦醇是葡萄浆果的关键分子成分，影响葡萄产品的质量。本工作的目的是鉴定 37 种经基因认证的拉蒂亚Latial 葡萄品种的代谢谱。特别是通过 LC-MS 和分光光度分析，对特定次生代谢物和总酚和单宁含量进行了定性和定量分析。此外，由于植物分子以其自由基清除特性而闻名，因此通过两种不同的清除自由基试验(DPPH 和 FRAP 试验)评估了样品提取物的抗氧化作用。最后，对每个标本对人结肠癌细胞 HCT-116 的抗增殖活性进行了初步筛选。所有结果表明，所有品种中都存在大量的酚类化合物;此外，我们还观察到提取物中代谢物浓度和生物活性之间存在显著相关性。此外，一些样品表现出非凡的生物学效应，例如与细胞毒性无关的肿瘤细胞生长减少，这支持了它们作为癌症治疗可能的辅助剂的使用。总之，本研究增加了对意大利本土葡萄生态型的科学认识，以提高其价值，并支持它们重新引入当地经济体系。
