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2
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Scand J Clin Lab Invest. 2011 May;71(3):221-6. doi: 10.3109/00365513.2011.554996. Epub 2011 Feb 3.
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Typical leptin fall is mitigated by breastfeeding in female infants.女性婴儿母乳喂养可减轻瘦素的典型下降。
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Evaluation of a multiplex assay for adipokine concentrations in obese children.肥胖儿童脂肪因子浓度的多重分析评估。
Clin Chem Lab Med. 2010 Oct;48(10):1439-46. doi: 10.1515/CCLM.2010.276. Epub 2010 Jun 25.
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A multiplex immunoassay for human adipokine profiling.一种用于人类脂肪因子分析的多重免疫分析法。
Clin Chem. 2010 Aug;56(8):1320-8. doi: 10.1373/clinchem.2010.146118. Epub 2010 Jun 8.
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Validity of multiplex-based assays for cytokine measurements in serum and plasma from "non-diseased" subjects: comparison with ELISA.基于多重检测法检测“非患病”受试者血清和血浆中细胞因子的有效性：与酶联免疫吸附测定法的比较
J Immunol Methods. 2009 Oct 31;350(1-2):125-32. doi: 10.1016/j.jim.2009.09.001. Epub 2009 Sep 10.
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8
In situ profiling of adipokines in subcutaneous microdialysates from lean and obese individuals.对瘦人和肥胖者皮下微透析液中脂肪因子的原位分析。
Am J Physiol Endocrinol Metab. 2008 Nov;295(5):E1095-105. doi: 10.1152/ajpendo.90483.2008. Epub 2008 Sep 9.
9
An evaluation of commercial fluorescent bead-based luminex cytokine assays.基于商业荧光微球的Luminex细胞因子检测的评估。
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Using the Bland-Altman method to measure agreement with repeated measures.采用Bland-Altman方法测量重复测量的一致性。
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在健康新生儿群体中采用放射免疫分析法（RIA）与微粒子免疫分析法（MIA）检测瘦素

Measurement of Leptin by RIA Versus MIA in a Population of Healthy Newborns.

作者信息

Treviño-Garza Consuelo, Mancillas-Adame Leonardo, Estrada-Zúñiga Cynthia M, Villarreal-Pérez Jesús Z, Villarreal-Martinez Laura, De la O-Cavazos Manuel E

机构信息

Department of Pediatrics, Autonomous University of Nuevo Leon, Monterrey, Mexico.

Division of Endocrinology, Autonomous University of Nuevo Leon, Monterrey, Mexico.

出版信息

J Clin Lab Anal. 2016 May;30(3):254-7. doi: 10.1002/jcla.21845. Epub 2015 Mar 26.

DOI:10.1002/jcla.21845
PMID:25821014
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6806735/
Abstract

BACKGROUND

Assays based on multiplex immunoassay (MIA) technology have demonstrated advantages over enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA). Its acceptance depends on how well it performs in comparison to older techniques. The aim is to compare the results of leptin using RIA versus MIA.

METHODS

We analyzed 81 samples of umbilical cord blood of healthy term newborns by RIA and MIA.

RESULTS

The concordance correlation coefficient was 0.158 (95% CI 0.10-0.21). Pearson's correlation coefficient was 0.6651 (95% CI 0.52-0.77; P < 0.0001). In the Bland-Altman plot, concordance is acceptable because most of the measurements are within a mean of ±1.96 SD.

CONCLUSIONS

As shown by the Bland-Altman plot, there is concordance by both methods, but with a weak correlation.

摘要

背景

基于多重免疫分析（MIA）技术的检测方法已显示出优于酶联免疫吸附测定（ELISA）和放射免疫测定（RIA）的优势。其认可度取决于与旧技术相比的性能表现。目的是比较使用RIA和MIA检测瘦素的结果。

方法

我们采用RIA和MIA分析了81例健康足月儿的脐带血样本。

结果

一致性相关系数为0.158（95%可信区间0.10 - 0.21）。Pearson相关系数为0.6651（95%可信区间0.52 - 0.77；P < 0.0001）。在Bland - Altman图中，一致性是可接受的，因为大多数测量值在均值±1.96标准差范围内。

结论

如Bland - Altman图所示，两种方法存在一致性，但相关性较弱。