Nair Manitha, Nancy D, Krishnan Amit G, Anjusree G S, Vadukumpully Sajini, Nair Shantikumar V
Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences & Research Centre, Amrita Vishwa Vidyapeetham, Kochi-682041, Kerala, India.
Nanotechnology. 2015 Apr 24;26(16):161001. doi: 10.1088/0957-4484/26/16/161001. Epub 2015 Mar 31.
In this study, graphene oxide (GO) nanoflakes (0.5 and 1 wt%) were incorporated into a gelatin-hydroxyapatite (GHA) matrix through a freeze drying technique and its effect to enhance mechanical strength and osteogenic differentiation was studied. The GHA matrix with GO demonstrated less brittleness in comparison to GHA scaffolds. There was no significant difference in mechanical strength between GOGHA0.5 and GOGHA1.0 scaffolds. When the scaffolds were immersed in phosphate buffered saline (to mimic physiologic condition) for 60 days, around 50-60% of GO was released in sustained and linear manner and the concentration was within the toxicity limit as reported earlier. Further, GOGHA0.5 scaffolds were continued for cell culture experiments, wherein the scaffold induced osteogenic differentiation of human adipose derived mesenchymal stem cells without providing supplements like dexamethasone, L-ascorbic acid and β glycerophosphate in the medium. The level of osteogenic differentiation of stem cells was comparable to those cultured on GHA scaffolds with osteogenic supplements. Thus biocompatible, biodegradable and porous GO reinforced gelatin-HA 3D scaffolds may serve as a suitable candidate in promoting bone regeneration in orthopaedics.
在本研究中，通过冷冻干燥技术将氧化石墨烯（GO）纳米片（0.5重量%和1重量%）掺入明胶-羟基磷灰石（GHA）基质中，并研究其对增强机械强度和成骨分化的影响。与GHA支架相比，含有GO的GHA基质脆性更小。GOGHA0.5和GOGHA1.0支架之间的机械强度没有显著差异。当支架浸入磷酸盐缓冲盐水（以模拟生理条件）60天时，约50-60%的GO以持续且线性的方式释放，且浓度在先前报道的毒性限度内。此外，继续对GOGHA0.5支架进行细胞培养实验，其中该支架在培养基中未添加地塞米松/L-抗坏血酸和β甘油磷酸等补充剂的情况下诱导了人脂肪来源间充质干细胞的成骨分化。干细胞的成骨分化水平与在添加了成骨补充剂的GHA支架上培养的细胞相当。因此，具有生物相容性、可生物降解且多孔的GO增强明胶-羟基磷灰石三维支架可能是促进骨科骨再生的合适候选材料。
