Luts A, Uddman R, Alm P, Basterra J, Sundler F
Department of Medical Cell Research, Malmö General Hospital, University of Lund, Sweden.
Int Arch Allergy Immunol. 1993;101(1):52-60. doi: 10.1159/000236498.
The occurrence, distribution and coexistence pattern of an array of neuropeptides and tyrosine hydroxylase in the human larynx, trachea, bronchi and lungs were studied by immunocytochemistry. A rich supply of nerve fibers containing vasoactive intestinal peptide (VIP) was seen close to blood vessels, glands and nonvascular smooth muscle. Pituitary adenylate cyclase-activating peptide (PACAP)-containing fibers were numerous among bundles of smooth muscle. Moderate numbers of helospectin-containing nerve fibers were seen in the nonvascular smooth muscle. The majority of neuropeptide Y (NPY)-containing fibers were located in the nonvascular smooth muscle; some fibers also occurred around blood vessels and glands. Substance P (SP) and calcitonin gene-related peptide (CGRP)-containing fibers were generally few and distributed beneath the epithelium, among bundles of smooth muscle, around blood vessels and glands. A conspicuous finding was the lack of SP- and CGRP-containing fibers within the respiratory epithelium. Galanin-containing nerve fibers were moderate in number among bundles of smooth muscle. Tyrosine hydroxylase-containing fibers were numerous around blood vessels and glands. The majority of the VIP-containing nerve fibers present in nonvascular smooth muscle also stored PACAP and helospectin. A subpopulation of VIP-containing fibers in both vascular and nonvascular smooth muscle and around glands stored NPY. Additionally, galanin was found to occur in many VIP-containing fibers located among bundles of smooth muscle.
采用免疫细胞化学方法研究了一系列神经肽和酪氨酸羟化酶在人喉、气管、支气管和肺中的发生、分布及共存模式。在血管、腺体和非血管平滑肌附近可见丰富的含血管活性肠肽（VIP）的神经纤维。含垂体腺苷酸环化酶激活肽（PACAP）的纤维在平滑肌束中数量众多。在非血管平滑肌中可见中等数量的含海洛辛的神经纤维。大多数含神经肽Y（NPY）的纤维位于非血管平滑肌中；一些纤维也出现在血管和腺体周围。含P物质（SP）和降钙素基因相关肽（CGRP）的纤维通常较少，分布于上皮下方、平滑肌束之间、血管和腺体周围。一个显著的发现是呼吸上皮内缺乏含SP和CGRP的纤维。含甘丙肽的神经纤维在平滑肌束中数量中等。含酪氨酸羟化酶的纤维在血管和腺体周围数量众多。存在于非血管平滑肌中的大多数含VIP的神经纤维也储存PACAP和海洛辛。在血管和平滑肌以及腺体周围的含VIP纤维亚群中储存NPY。此外，发现甘丙肽存在于许多位于平滑肌束之间的含VIP纤维中。
