Rai Mahendra, Ingle Avinash P, Gade Aniket, Duran Nelson
Biological Chemistry Laboratory, Institute of Chemistry, Universidade Estadual de Campinas, Campinas, SP, Brazil.
Department of Biotechnology, SGB Amravati University, Amravati-444 602, Maharashtra, India.
IET Nanobiotechnol. 2015 Apr;9(2):71-5. doi: 10.1049/iet-nbt.2014.0013.
The authors report the biological synthesis of silver nanoparticles (NPs) by Phoma gardeniae (ITCC 4554). The detection of silver NP formation was done by visual observation, and UV-vis spectrophotometer analysis. Further, these mycogenic silver NPs were characterised by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and nanoparticle tracking and analysis system. The TEM analysis revealed the formation of spherical and polydispersed NPs within the range of 10-30 nm. FTIR analysis confirmed the presence of proteins as capping agents. They also evaluated the antimicrobial activity of silver NPs against Candida albicans, Salmonella choleraesuis, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. They found remarkable inhibition of Escherichia coli followed by Staphylococcus aureus, Candida albicans, Salmonella choleraesuis and Pseudomonas aeruginosa as compared with antibiotics. The main aim of the present study was to synthesise mycogenic silver NPs by P. gardeniae and to evaluate their antimicrobial activity in order to find their potential against human pathogenic microbes.
作者报道了由栀子茎点霉（ITCC 4554）生物合成银纳米颗粒（NPs）。通过肉眼观察和紫外可见分光光度计分析对银纳米颗粒的形成进行检测。此外，利用傅里叶变换红外光谱（FTIR）、透射电子显微镜（TEM）和纳米颗粒跟踪分析系统对这些真菌源银纳米颗粒进行表征。透射电子显微镜分析显示形成了尺寸在10 - 30纳米范围内的球形且多分散的纳米颗粒。傅里叶变换红外光谱分析证实存在作为封端剂的蛋白质。他们还评估了银纳米颗粒对白色念珠菌、猪霍乱沙门氏菌、铜绿假单胞菌、金黄色葡萄球菌和大肠杆菌的抗菌活性。与抗生素相比，他们发现银纳米颗粒对大肠杆菌有显著抑制作用，其次是金黄色葡萄球菌、白色念珠菌、猪霍乱沙门氏菌和铜绿假单胞菌。本研究的主要目的是通过栀子茎点霉合成真菌源银纳米颗粒并评估其抗菌活性，以发现其对人类致病微生物的潜在作用。
