Larguinho Miguel, Santos Sofia, Almeida João, Baptista Pedro V
REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal.
CIGMH, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal.
IET Nanobiotechnol. 2015 Apr;9(2):95-101. doi: 10.1049/iet-nbt.2014.0007.
The optical and physico-chemical properties of gold nanoparticles (AuNPs) have prompted new and improved approaches which have greatly evolved the fields of biosensing and molecular detection. In this study, the authors took advantage of AuNPs' ease of modification and functionalised it with selected DNA aptamers using a salt aging method to produce gold-aptamer nanoprobes. After characterisation, these nanoprobes were subsequently used for biomolecular detection of glycidamide (GA)-guanine (Gua) adducts generated in vitro. The results are based on differences in nanoprobe stabilisation against salt-induced aggregation, similar to the non-cross-linking method developed by Baptista for discrimination of specific sequences. Alkylated Guas were efficiently discriminated from deoxyguanosine and GA in solution. Despite this, a clear identification of DNA adducts derived from genomic DNA alkylation has proven to be a more challenging task.
金纳米颗粒（AuNPs）的光学和物理化学性质催生了新的、改进的方法，极大地推动了生物传感和分子检测领域的发展。在本研究中，作者利用AuNPs易于修饰的特点，采用盐老化法用选定的DNA适体对其进行功能化，以制备金适体纳米探针。经过表征后，这些纳米探针随后被用于体外生成的缩水甘油酰胺（GA）-鸟嘌呤（Gua）加合物的生物分子检测。结果基于纳米探针针对盐诱导聚集的稳定性差异，类似于Baptista开发的用于区分特定序列的非交联方法。在溶液中，烷基化的鸟嘌呤能有效地与脱氧鸟苷和GA区分开来。尽管如此，明确鉴定源自基因组DNA烷基化的DNA加合物已被证明是一项更具挑战性的任务。
