根尖充填材料的生物学及化学物理特性：一项对比研究。
Biological and chemical-physical properties of root-end filling materials: A comparative study.
作者信息
Ceci Matteo, Beltrami Riccardo, Chiesa Marco, Colombo Marco, Poggio Claudio
机构信息
Departments of Clinical-Surgical, Diagnostic and Pediatric Sciences - Sections of Dentistry, University of Pavia, Pavia, Italy.
Brain and Behavioral, Sections of Statistics, University of Pavia, Pavia, Italy.
出版信息
J Conserv Dent. 2015 Mar-Apr;18(2):94-9. doi: 10.4103/0972-0707.153058.
AIM
The purpose of the study is to evaluate and compare the biological and chemical-physical properties of four different root-end filling materials.
MATERIALS AND METHODS
Cytotoxicity towards murine odontoblasts cells (MDPC-23) was evaluated using the Transwell insert methodology by Alamar blue test. Streptococcus salivarius, S. sanguis, and S. mutans strains were selected to evaluate the antimicrobial activity by agar disc diffusion test. Solubility was determined after 24 h and 2 months. pH values were measured after 3 and 24 h. To evaluate radiopacity, all materials were scanned on a GE Healthcare Lunar Prodigy.
RESULTS
Excellent percentage of vitality were obtained by mineral trioxide aggregate (MTA)-based materials and Biodentine. MTA-Angelus, ProRoot MTA, and Intermediate Restorative Material (IRM) showed the highest values for the inhibition zones when tested for S. mutans, while Biodentine showed the largest inhibition zone when tested for S. sanguis. All the materials fulfilled the requirements of the International Standard 6876, demonstrating low solubility with a weight loss of less than 3%. No significant reduction in pH value was demonstrated after 24 h. ProRoot MTA and MTA-Angelus showed the highest values of radiographic density.
CONCLUSIONS
The differences showed by the root-end filling materials tested do not cover completely the ideal clinical requests.
目的
本研究旨在评估和比较四种不同根尖充填材料的生物学和化学物理性能。
材料与方法
采用Transwell小室法通过阿拉玛蓝试验评估对小鼠成牙本质细胞（MDPC-23）的细胞毒性。选择唾液链球菌、血链球菌和变形链球菌菌株，通过琼脂扩散试验评估抗菌活性。在24小时和2个月后测定溶解度。在3小时和24小时后测量pH值。为评估射线不透性，所有材料均在GE医疗Lunar Prodigy上进行扫描。
结果
基于三氧化矿物凝聚体（MTA）的材料和生物陶瓷获得了优异的活力百分比。当检测变形链球菌时，MTA-Angelus、ProRoot MTA和中间修复材料（IRM）显示出最高的抑菌圈值，而当检测血链球菌时，生物陶瓷显示出最大的抑菌圈。所有材料均符合国际标准6876的要求，显示出低溶解度，重量损失小于3%。24小时后pH值无显著降低。ProRoot MTA和MTA-Angelus显示出最高的射线密度值。
结论
所测试的根尖充填材料所显示的差异并未完全满足理想的临床要求。
Zotero 插件