Bossù Maurizio, Mancini Patrizia, Bruni Erika, Uccelletti Daniela, Preziosi Adele, Rulli Marco, Relucenti Michela, Donfrancesco Orlando, Iaculli Flavia, Di Giorgio Gianni, Matassa Roberto, Salucci Alessandro, Polimeni Antonella
Department of Oral and Maxillofacial Science, Sapienza University of Rome, Via Caserta 6, 00161 Rome, Italy.
Department of Experimental Medicine, Sapienza University of Rome, Viale Regina Elena 324, 00161 Rome, Italy.
Biology (Basel). 2021 May 26;10(6):470. doi: 10.3390/biology10060470.
Calcium silicate-based cements have reached excellent levels of performance in endodontics, providing predictable and successful results. To better assess the properties of these bioactive materials, the present study aimed to compare the biocompatibility and antibiofilm properties of ProRoot MTA and Biodentine. Human osteogenic sarcoma (Saos-2) cells were cultured on ProRoot MTA and Biodentine samples or in the presence of both cement extracts. Cell viability assay, measurement of reactive oxygen species (ROS), immunofluorescence analysis, as well as morphological evaluations were conducted. Moreover, was used to assess the biofilm forming ability on ProRoot MTA and Biodentine disks. Finally, both cements were applied in vivo to treat immature permanent teeth affected by reversible pulpitis. Results: Cell viability assay demonstrated that Saos-2 cells had a dose- and time-dependent cytotoxicity to both analyzed cements, although cells exposed to ProRoot MTA showed a better cell vitality than those exposed to Biodentine ( < 0.001). Both cements demonstrated ROS production while this was greater in the case of Biodentine than ProRoot MTA ( < 0.001). Immunofluorescence images of the cytoskeleton and focal adhesions showed no differences in Saos-2 cells grown in the presence of ProRoot MTA eluate; whereas in the Biodentine groups, cells showed a morphology and focal adhesions more similar to that of the control sample, as the eluate concentration decreased. Morphological analysis revealed that Saos-2 cells were more flattened and exhibited better spreading when attached to ProRoot MTA disks than to Biodentine ones. The antibiofilm properties showed a time-dependent powerful inhibition of superficial colonization and an antibiofilm effect of both cements. Clinically, complete root formation of the treated elements was achieved using the two studied cements, showing stable results over time. ProRoot MTA and Biodentine was demonstrated to be biocompatible and to possess antibiofilm properties. Their clinical application in vital pulp therapy provided successful outcomes after 2 years of follow-up.
硅酸钙基水门汀在牙髓病学领域已达到优异的性能水平，能提供可预测且成功的治疗效果。为了更好地评估这些生物活性材料的性能，本研究旨在比较ProRoot MTA和Biodentine的生物相容性及抗生物膜特性。将人骨肉瘤（Saos-2）细胞培养在ProRoot MTA和Biodentine样本上，或在两种水门汀提取物存在的情况下进行培养。进行了细胞活力测定、活性氧（ROS）测量、免疫荧光分析以及形态学评估。此外，还用于评估ProRoot MTA和Biodentine圆盘上的生物膜形成能力。最后，将两种水门汀应用于体内治疗受可逆性牙髓炎影响的年轻恒牙。结果：细胞活力测定表明，Saos-2细胞对两种分析的水门汀均具有剂量和时间依赖性细胞毒性，尽管暴露于ProRoot MTA的细胞比暴露于Biodentine的细胞显示出更好的细胞活力（<0.001）。两种水门汀均显示出ROS产生，而Biodentine的ROS产生量大于ProRoot MTA（<0.001）。细胞骨架和粘着斑的免疫荧光图像显示，在ProRoot MTA洗脱液存在下生长的Saos-2细胞无差异；而在Biodentine组中，随着洗脱液浓度降低，细胞显示出与对照样本更相似的形态和粘着斑。形态学分析表明，Saos-2细胞附着在ProRoot MTA圆盘上时比附着在Biodentine圆盘上更扁平且铺展更好。抗生物膜特性显示出对表面定植的时间依赖性强力抑制以及两种水门汀的抗生物膜作用。临床上，使用这两种研究的水门汀实现了治疗牙齿的完全牙根形成，且随着时间推移结果稳定。ProRoot MTA和Biodentine被证明具有生物相容性并具有抗生物膜特性。它们在活髓治疗中的临床应用在2年随访后取得了成功的结果。
