• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

黑种草诱导MCF和HepG2细胞系生长抑制的研究：一项抗肿瘤研究及其作用机制。

A study of Nigella sativa induced growth inhibition of MCF and HepG2 cell lines: An anti-neoplastic study along with its mechanism of action.

作者信息

Reddy Y Padmanabha, Chandrasekhar K B, Sadiq Mohammed Jaffar

机构信息

Department of Pharmaceutcal Analysis, Raghavendra Institute of Pharmaceutical Education and Research, Andhra Pradesh, India.

Department of Chemistry, JNTUA, Ananthapuramu, Andhra Pradesh, India.

出版信息

Pharmacognosy Res. 2015 Apr-Jun;7(2):193-7. doi: 10.4103/0974-8490.150541.

DOI:10.4103/0974-8490.150541
PMID:25829794
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4357971/
Abstract

OBJECTIVE

To evaluate the anticancer potential of seeds of Nigella sativa using MCF and HepG2 cell lines along with its mechanism of action.

MATERIALS AND METHODS

(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and acridine orange/ethidium bromide nuclear staining technique were selected to evaluate anticancer potential and mechanism of action of test extract.

RESULTS

Aqueous extract of N.sativa at a test dose of 180 mg and 300 mg was identified to be the best as anticancer agent against MCF and HepG2 cell lines among different solvent test extract where doxorubicin and cisplatin were employed as standard references.

DISCUSSION

Further study including separation and characterization of active principles in the aqueous extract shall prove beneficial.

摘要

目的

利用MCF和HepG2细胞系评估黑种草籽的抗癌潜力及其作用机制。

材料与方法

选用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法和吖啶橙/溴化乙锭核染色技术来评估受试提取物的抗癌潜力和作用机制。

结果

在以阿霉素和顺铂作为标准对照的不同溶剂受试提取物中，黑种草籽水提取物在180毫克和300毫克的测试剂量下被确定为针对MCF和HepG2细胞系的最佳抗癌剂。

讨论

包括对水提取物中活性成分进行分离和表征在内的进一步研究将证明是有益的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1607/4357971/3b743ac76e4c/PR-7-193-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1607/4357971/75b9c55b43ec/PR-7-193-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1607/4357971/38e886817b65/PR-7-193-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1607/4357971/ffa15df00e87/PR-7-193-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1607/4357971/3b743ac76e4c/PR-7-193-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1607/4357971/75b9c55b43ec/PR-7-193-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1607/4357971/38e886817b65/PR-7-193-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1607/4357971/ffa15df00e87/PR-7-193-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1607/4357971/3b743ac76e4c/PR-7-193-g005.jpg

相似文献

1
A study of Nigella sativa induced growth inhibition of MCF and HepG2 cell lines: An anti-neoplastic study along with its mechanism of action.黑种草诱导MCF和HepG2细胞系生长抑制的研究：一项抗肿瘤研究及其作用机制。
Pharmacognosy Res. 2015 Apr-Jun;7(2):193-7. doi: 10.4103/0974-8490.150541.
2
The effect of hydro alcoholic extract of Nigella sativa seeds on inflammatory mediators in C6 glioma cell line.黑种草籽水醇提取物对C6胶质瘤细胞系中炎症介质的影响。
Ann Pharm Fr. 2023 May;81(3):446-456. doi: 10.1016/j.pharma.2022.10.002. Epub 2022 Oct 14.
3
Synergistic anti-cancer effects of seed oil and conventional cytotoxic agent against human breast cancer.籽油与常规细胞毒药物协同抗人乳腺癌作用。
Drug Metab Pers Ther. 2022 Apr 11;37(3):315-321. doi: 10.1515/dmpt-2021-0229. eCollection 2022 Sep 1.
4
Hormetic/cytotoxic effects of Nigella sativa seed alcoholic and aqueous extracts on MCF-7 breast cancer cells alone or in combination with doxorubicin.黑种草籽的醇提物和水提物单独或联合阿霉素对 MCF-7 乳腺癌细胞的抗细胞毒性作用。
Cell Biochem Biophys. 2013 Jul;66(3):451-60. doi: 10.1007/s12013-012-9493-4.
5
Unveiling the therapeutic potential of thymol from Nigella sativa L. seed: selective anticancer action against human breast cancer cells (MCF-7) through down-regulation of Cyclin D1 and proliferative cell nuclear antigen (PCNA) expressions.揭示黑种草籽油中麝香草酚的治疗潜力：通过下调细胞周期蛋白 D1 和增殖细胞核抗原（PCNA）的表达，对人乳腺癌细胞（MCF-7）具有选择性抗癌作用。
Mol Biol Rep. 2024 Jan 3;51(1):61. doi: 10.1007/s11033-023-09032-w.
6
A comparison of the cytotoxic potential of standardized aqueous and ethanolic extracts of a polyherbal mixture comprised of Nigella sativa (seeds), Hemidesmus indicus (roots) and Smilax glabra (rhizome).对由黑种草（种子）、印度牛弥菜（根）和光叶菝葜（根茎）组成的多草药混合物的标准化水提取物和乙醇提取物的细胞毒性潜力进行比较。
Pharmacognosy Res. 2010 Nov;2(6):335-42. doi: 10.4103/0974-8490.75451.
7
Antipsoriatic activity and cytotoxicity of ethanolic extract of Nigella sativa seeds.黑种草籽乙醇提取物的抗银屑病活性和细胞毒性。
Pharmacogn Mag. 2012 Oct;8(32):268-72. doi: 10.4103/0973-1296.103650.
8
Anticancer Property of Digera muricata Leaf Extract: An In Vitro Study.地锦叶提取物的抗癌特性：一项体外研究
Cureus. 2023 Nov 23;15(11):e49276. doi: 10.7759/cureus.49276. eCollection 2023 Nov.
9
Proapoptotic and antimetastatic properties of supercritical CO2 extract of Nigella sativa Linn. against breast cancer cells.黑种草超临界二氧化碳提取物对乳腺癌细胞的促凋亡和抗转移特性。
J Med Food. 2013 Dec;16(12):1121-30. doi: 10.1089/jmf.2012.2624.
10
Nigella sativa L. seed extract alleviates oxidative stress-induced cellular senescence and dysfunction in melanocytes.
Chin J Nat Med. 2025 Feb;23(2):203-213. doi: 10.1016/S1875-5364(25)60824-7.

引用本文的文献

1
Neuraminidase as a novel therapeutic management strategy for Alzheimer's disease: evidenced through molecular docking, molecular dynamic simulation and gene expression analysis.神经氨酸酶作为阿尔茨海默病的一种新型治疗策略：通过分子对接、分子动力学模拟和基因表达分析得到证实。
Front Chem. 2025 May 30;13:1574702. doi: 10.3389/fchem.2025.1574702. eCollection 2025.
2
Targeting cell cycle arrest in breast cancer by phytochemicals from Caryto urens L. fruit ethyl acetate fraction: in silico and in vitro validation.利用刺桐果实乙酸乙酯部位的植物化学物质靶向乳腺癌细胞周期阻滞：计算机模拟和体外验证
J Ayurveda Integr Med. 2025 Mar 12;16(2):101095. doi: 10.1016/j.jaim.2024.101095.
3

本文引用的文献

1
Antiproliferative properties of methanolic extract of Nigella sativa against the MDA-MB-231 cancer cell line.黑种草甲醇提取物对MDA-MB-231癌细胞系的抗增殖特性。
Asian Pac J Cancer Prev. 2012;13(11):5839-42. doi: 10.7314/apjcp.2012.13.11.5839.
2
Pharmacognostic standardization, physico- and phytochemical evaluation of amaranthus spinosus linn. Root.刺苋根的生药学标准化、物理和植物化学评价
J Young Pharm. 2011 Jul;3(3):221-5. doi: 10.4103/0975-1483.83770.
3
The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay is a fast and reliable method for colorimetric determination of fungal cell densities.
Knowledge, Attitudes, and Practices Related to Cervical Cancer Prevention and Screening among Female Pharmacy Students at a Public University in a Southern Region of Saudi Arabia.
沙特阿拉伯南部地区一所公立大学女性药学专业学生中与宫颈癌预防和筛查相关的知识、态度和行为
Healthcare (Basel). 2023 Oct 22;11(20):2798. doi: 10.3390/healthcare11202798.
4
Rutamarin, an Active Constituent from Pers., Induced Apoptotic Cell Death in the HT29 Colon Adenocarcinoma Cell Line.芸香苦素，一种来自Pers.的活性成分，诱导HT29结肠腺癌细胞系发生凋亡性细胞死亡。
Pharmacogn Mag. 2017 Jul;13(Suppl 2):S179-S188. doi: 10.4103/pm.pm_432_16. Epub 2017 Jul 11.
5
North African Medicinal Plants Traditionally Used in Cancer Therapy.传统上用于癌症治疗的北非药用植物。
Front Pharmacol. 2017 Jun 26;8:383. doi: 10.3389/fphar.2017.00383. eCollection 2017.
6
Protective effect of Nigella sativa seeds against spermatocyte chromosomal aberrations and genotoxicity induced by carbon tetrachloride in mice.黑种草籽对四氯化碳诱导的小鼠精母细胞染色体畸变和遗传毒性的保护作用。
Environ Sci Pollut Res Int. 2017 Apr;24(12):11677-11682. doi: 10.1007/s11356-017-8806-y. Epub 2017 Mar 21.
MTT [3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐] 测定法是一种用于比色法测定真菌细胞密度的快速且可靠的方法。
Appl Environ Microbiol. 1999 Aug;65(8):3727-9. doi: 10.1128/AEM.65.8.3727-3729.1999.
4
A simple method for counting adherent cells: application to cultured human monocytes, macrophages and multinucleated giant cells.一种计数贴壁细胞的简单方法：应用于培养的人单核细胞、巨噬细胞和多核巨细胞。
J Immunol Methods. 1983 Jan 28;56(2):261-8. doi: 10.1016/0022-1759(83)90418-0.