Carney Christiane E, Lenov Ivan L, Baker Catherine J, MacRenaris Keith W, Eckermann Amanda L, Sligar Stephen G, Meade Thomas J
†Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
‡Department of Biochemistry, 505 South Goodwin Avenue, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.
Bioconjug Chem. 2015 May 20;26(5):899-905. doi: 10.1021/acs.bioconjchem.5b00107. Epub 2015 Apr 9.
Nanodiscs are monodisperse, self-assembled discoidal particles that consist of a lipid bilayer encircled by membrane scaffold proteins (MSP). Nanodiscs have been used to solubilize membrane proteins for structural and functional studies and deliver therapeutic phospholipids. Herein, we report on tetramethylrhodamine (TMR) tagged nanodiscs that solubilize lipophilic MR contrast agents for generation of multimodal nanoparticles for cellular imaging. We incorporate both multimeric and monomeric Gd(III)-based contrast agents into nanodiscs and show that particles containing the monomeric agent (ND2) label cells with high efficiency and generate significant image contrast at 7 T compared to nanodiscs containing the multimeric agent (ND1) and Prohance, a clinically approved contrast agent.
纳米盘是单分散的自组装盘状颗粒，由被膜支架蛋白（MSP）环绕的脂质双层组成。纳米盘已被用于溶解膜蛋白以进行结构和功能研究，并递送治疗性磷脂。在此，我们报道了用四甲基罗丹明（TMR）标记的纳米盘，其可溶解亲脂性磁共振造影剂以生成用于细胞成像的多模态纳米颗粒。我们将基于多聚体和单体的钆（III）造影剂都整合到纳米盘中，并表明含有单体造影剂的颗粒（ND2）能高效标记细胞，且与含有多聚体造影剂的纳米盘（ND1）和临床批准的造影剂普乐显相比，在7T时能产生显著的图像对比度。
