Carney Christiane E, MacRenaris Keith W, Meade Thomas J
Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, Evanston, IL, 60208-3113, USA.
J Biol Inorg Chem. 2015 Sep;20(6):971-7. doi: 10.1007/s00775-015-1280-4. Epub 2015 Jul 28.
Long-term cell tracking using MR imaging necessitates the development of contrast agents that both label and are retained by cells. One promising strategy for long-term cell labeling is the development of lipophilic Gd(III)-based contrast agents that anchor into the cell membrane. We have previously reported the efficacy of monomeric and multimeric lipophilic agents and showed that the monomeric agents have improved labeling and contrast enhancement of cell populations. Here, we report on the synthesis, characterization, and in vitro testing of a series of monomeric lipophilic contrast agents with varied alkyl chain compositions. We show that these agents disperse in water, localize to the cell membrane, and label HeLa and MCF7 cells effectively. Additionally, these agents have up to tenfold improved retention in cells compared to clinically available ProHance(®).
使用磁共振成像进行长期细胞追踪需要开发既能标记细胞又能被细胞保留的造影剂。一种有前景的长期细胞标记策略是开发锚定在细胞膜上的亲脂性钆（III）基造影剂。我们之前报道了单体和亲脂性多聚体造影剂的功效，并表明单体造影剂对细胞群体的标记和对比度增强效果更佳。在此，我们报告了一系列具有不同烷基链组成的单体亲脂性造影剂的合成、表征及体外测试。我们发现这些造影剂能分散于水中，定位于细胞膜，并有效标记HeLa细胞和MCF7细胞。此外，与临床可用的普美显（ProHance®）相比，这些造影剂在细胞中的保留率提高了多达10倍。
