Rauvala H
Department of Medical Chemistry, University of Helsinki, Finland.
EMBO J. 1989 Oct;8(10):2933-41. doi: 10.1002/j.1460-2075.1989.tb08443.x.
An 18-kd heparin-binding protein (p18) was isolated from perinatal rat brain. Although the protein closely resembles the fibroblast growth factors in its strong binding to heparin and in its apparent molecular mass, it has a distinct structure. This was concluded from the amino-terminal sequence analysis that identified a unique structure containing a cluster of lysine residues. Antipeptide antibodies were raised in rabbits according to the sequence analysis and affinity purified using a synthetic peptide. The antibodies were shown to bind specifically to p18, which was immunochemically distinct from the basic fibroblast growth factor. The antipeptide antibodies detected p18 in brain but not in liver, kidney, heart or skeletal muscle. The content of the protein was shown to undergo a remarkable developmental change corresponding to the time period of rapid sprouting of axons and dendrites in brain. The content of p18 was rapidly increased at the time of birth until the postnatal age of approximately 1 week, after which it was decreased to values less than 10% in young adults as compared to the content found in perinatal rats. p18 also enhanced neurite outgrowth in brain neurons in vitro. The protein was stained in neurons in cells dispersed from perinatal brain. The properties of p18 suggest that it has a role in the growth and maturation of brain.
从围产期大鼠脑部分离出一种18-kd的肝素结合蛋白（p18）。尽管该蛋白在与肝素的强结合能力和表观分子量方面与成纤维细胞生长因子极为相似，但其结构独特。这一结论是通过氨基末端序列分析得出的，该分析鉴定出一种含有赖氨酸残基簇的独特结构。根据序列分析在兔体内制备了抗肽抗体，并使用合成肽进行亲和纯化。结果表明，这些抗体能特异性结合p18，p18在免疫化学上与碱性成纤维细胞生长因子不同。抗肽抗体在脑中能检测到p18，但在肝脏、肾脏、心脏或骨骼肌中未检测到。结果显示，该蛋白的含量经历了显著的发育变化，这与脑内轴突和树突快速萌发的时间段相对应。p18的含量在出生时迅速增加，直至出生后约1周龄，之后与围产期大鼠相比，在年轻成年大鼠中降至不到10%。p18在体外也能增强脑神经元的神经突生长。该蛋白在从围产期脑部分散的细胞中的神经元中被染色。p18的特性表明它在脑的生长和成熟过程中发挥作用。
