Jackson Stephen A, Kennedy Jonathan, Morrissey John P, O'Gara Fergal, Dobson Alan D W
Marine Biotechnology Centre, Environmental Research Institute, University College Cork, Cork, Ireland.
School of Microbiology, University College Cork, Cork, Ireland.
Int J Syst Evol Microbiol. 2015 Jul;65(7):2097-2103. doi: 10.1099/ijs.0.000224. Epub 2015 Apr 1.
Two Gram-stain-negative, rod-shaped, orange, catalase- and oxidase-positive, non-motile bacteria, designated W13M1A(T) and W15M10(T), were isolated from the marine sponges Suberites carnosus and Leucosolenia sp., respectively, which were sampled from Lough Hyne, Co. Cork, Ireland. Analysis of the 16S rRNA gene sequences of these isolates revealed that they are members of the genus Maribacter, in the family Flavobacteriaceae of the phylum Bacteroidetes. The type strain most closely related to strain W13M1A(T) is Maribacter forsetii DSM 18668(T) with a gene sequence similarity of 96.5%. The closest related type strain to strain W15M10(T) is Maribacter orientalis DSM 16471(T) with a gene sequence similarity of 98.3%. Phylogenetic inference and phenotypic data combined indicate that the isolates represent two novel species of the genus Maribacter, for which the names Maribacter spongiicola sp. nov. with type strain W15M10(T) ( = NCIMB 14725(T) = DSM 25233(T)) and Maribacter vaceletii sp. nov. with type strain W13M1A(T) ( = NCIMB 14724(T) = DSM 25230(T)), are proposed.
从爱尔兰科克郡海恩湖采集的海洋海绵肉海绵（Suberites carnosus）和白枝海绵属（Leucosolenia sp.）中分别分离出两株革兰氏阴性、杆状、橙色、过氧化氢酶和氧化酶阳性、无运动性的细菌，分别命名为W13M1A(T)和W15M10(T)。对这些分离株的16S rRNA基因序列分析表明，它们是拟杆菌门黄杆菌科海杆菌属（Maribacter）的成员。与菌株W13M1A(T)最密切相关的模式菌株是福氏海杆菌（Maribacter forsetii）DSM 18668(T)，基因序列相似性为96.5%。与菌株W15M10(T)最密切相关的模式菌株是东方海杆菌（Maribacter orientalis）DSM 16471(T)，基因序列相似性为98.3%。系统发育推断和表型数据综合表明，这些分离株代表海杆菌属的两个新物种，为此提出了新物种海绵海杆菌（Maribacter spongiicola sp. nov.），模式菌株为W15M10(T)（=NCIMB 14725(T)=DSM 25233(T)）和瓦氏海杆菌（Maribacter vaceletii sp. nov.），模式菌株为W13M1A(T)（=NCIMB 14724(T)=DSM 25230(T)）。
