Marine College, Shandong University, Weihai, Shandong 264209, PR China.
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, PR China.
Int J Syst Evol Microbiol. 2020 Jun;70(6):3679-3685. doi: 10.1099/ijsem.0.004220. Epub 2020 May 15.
A Gram-stain-negative, rod-shaped (0.2-0.3×1.0-2.4 µm), catalase-positive, oxidase-negative and non-motile bacterium, designated strain RZ26, was isolated from the marine red algae collected from the coast of Weihai, PR China. Growth of strain RZ26 occurred at 15-33 °C (optimum, 25-28 °C), pH 6.0-9.5 (optimum, pH 7.0-7.5) and 0.5-5.0 % (w/v) NaCl (optimum, 2.0-3.0 %). Resuls of phylogenetic analysis based on 16S rRNA gene sequences showed that strain RZ26 was most closely related to DSM 25233 (96.2 % sequence similarity), followed by DSM 18668 (96.1 %) and DSM 25230 (95.4 %). The average nucleotide identity and the average amino acid identity values between strain RZ26 and KCTC 12966, DSM 25233, DSM 25230 and DSM 18668 were 75.6, 76.2, 76.0, 76.7, 64.3, 63.9, 68.6 and 68.0 %, respectively. The digital DNA-DNAhybridization values based on the draft genomes between strain RZ26 and KCTC 12966, DSM 25233 and DSM 25230 were 38.0, 35.1 and 37.1 %, respectively. The major fatty acids in strain RZ26 were iso-C 3-OH, iso-C and C 7/C 6. The major respiratory quinone was MK-6. The dominant polar lipid was phosphatidylethanolamine. The DNA G+C content was 38.0 mol%. Phylogenetic analysis shows strain RZ26 fell within a clade comprising species of the genus . Polyphasic taxonomy indicates that the isolate represents a novel species of the genus , for which the name sp. nov. is proposed, with type strain RZ26 (=KCTC 62992=MCCC 1H00362).
一株革兰氏阴性、杆状（0.2-0.3×1.0-2.4μm）、过氧化氢酶阳性、氧化酶阴性、不运动的细菌，被命名为 RZ26 菌株，从中国威海海岸采集的海洋红藻中分离得到。RZ26 菌株的生长温度为 15-33°C（最佳温度为 25-28°C），pH 值为 6.0-9.5（最佳 pH 值为 7.0-7.5），盐度为 0.5-5.0%（w/v）（最佳盐度为 2.0-3.0%）。基于 16S rRNA 基因序列的系统发育分析结果表明，菌株 RZ26 与 DSM 25233（96.2%序列相似性）最为密切相关，其次是 DSM 18668（96.1%）和 DSM 25230（95.4%）。菌株 RZ26 与 KCTC 12966、DSM 25233、DSM 25230 和 DSM 18668 的平均核苷酸同一性和平均氨基酸同一性值分别为 75.6%、76.2%、76.0%、76.7%、64.3%、63.9%、68.6%和 68.0%。基于 RZ26 菌株和 KCTC 12966、DSM 25233 和 DSM 25230 的基因组草图的数字 DNA-DNA 杂交值分别为 38.0%、35.1%和 37.1%。菌株 RZ26 的主要脂肪酸为 iso-C 3-OH、iso-C 和 C 7/C 6。主要呼吸醌为 MK-6。主要极性脂为磷脂酰乙醇胺。DNA G+C 含量为 38.0mol%。系统发育分析表明，菌株 RZ26 属于. 多相分类学表明，该分离株代表了属的一个新种，建议使用新种名称 sp. nov.，其模式菌株为 RZ26 菌株（=KCTC 62992=MCCC 1H00362）。
