Molecular Biosensing for Medical Diagnostics, Faculty of Applied Physics and Faculty of Biomedical Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600 MB, Eindhoven, The Netherlands.
Nano Lett. 2015 May 13;15(5):3507-11. doi: 10.1021/acs.nanolett.5b00872. Epub 2015 Apr 6.
We present a plasmonic biosensor based on hundreds of individual gold nanorods with single-molecule sensitivity that are simultaneously monitored in real-time within a dark-field microscopy setup. The approach allows for the statistical analysis of single-molecule interactions without requiring any labeling of the analyte. We study an antibody-antigen interaction and find that the waiting-time distribution is concentration-dependent and obeys Poisson statistics. The ability to probe hundreds of nanoparticles simultaneously will provide a sensor with a dynamic range of 7 decades in concentration and will enable the study of heterogeneity in molecular interactions.
我们提出了一种基于数百个具有单分子灵敏度的单个金纳米棒的等离子体生物传感器，这些纳米棒在暗场显微镜设置中实时同时进行监测。该方法允许在不标记分析物的情况下对单分子相互作用进行统计分析。我们研究了抗体-抗原相互作用，发现等待时间分布与浓度有关，符合泊松统计。同时探测数百个纳米粒子的能力将为传感器提供 7 个数量级的浓度动态范围，并能够研究分子相互作用中的异质性。
