Peterson D, Brenu E W, Gottschalk G, Ramos S, Nguyen T, Staines D, Marshall-Gradisnik S
Simmaron Research, 948 Incline Way, Incline Village, NV 89451, USA.
Griffith Health Institute, School of Medial Sciences, National Centre for Neuroimmunology and Emerging Diseases, Griffith University, Parklands, QLD 4222, Australia.
Mediators Inflamm. 2015;2015:929720. doi: 10.1155/2015/929720. Epub 2015 Mar 5.
Previous research has provided evidence for dysregulation in peripheral cytokines in patients with Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). To date only one study has examined cytokines in cerebrospinal fluid (CSF) samples of CFS/ME patients. The purpose of this pilot study was to examine the role of cytokines in CSF of CFS/ME patients.
CSF was collected from 18 CFS/ME patients and 5 healthy controls. The CSF samples were examined for the expression of 27 cytokines (interleukin- (IL-) 1β, IL-1ra, IL-2, IL-4, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17, basic FGF, eotaxin, G-CSF, GM-CSF, IFN-γ, IP-10, MCP-1 (MCAF), MIP-1α, MIP-1β, PDGF-BB, RANTES, TNF-α, and VEGF) using the Bio-Plex Human Cytokine 27-plex Assay.
Of the 27 cytokines examined, only IL-10 was significantly reduced in the CFS/ME patients in comparison to the controls.
This preliminary investigation suggests that perturbations in inflammatory cytokines in the CSF of CFS/ME patients may contribute to the neurological discrepancies observed in CFS/ME.
先前的研究已为慢性疲劳综合征/肌痛性脑脊髓炎（CFS/ME）患者外周细胞因子失调提供了证据。迄今为止，仅有一项研究检测了CFS/ME患者脑脊液（CSF）样本中的细胞因子。这项初步研究的目的是检测细胞因子在CFS/ME患者脑脊液中的作用。
收集了18例CFS/ME患者和5名健康对照者的脑脊液。使用Bio-Plex人类细胞因子27项检测法检测脑脊液样本中27种细胞因子（白细胞介素-（IL-）1β、IL-1ra、IL-2、IL-4、IL-6、IL-7、IL-8、IL-9、IL-10、IL-12p70、IL-13、IL-15、IL-17、碱性成纤维细胞生长因子、嗜酸性粒细胞趋化因子、粒细胞集落刺激因子、粒细胞-巨噬细胞集落刺激因子、干扰素-γ、IP-10、单核细胞趋化蛋白-1（MCAF）、巨噬细胞炎性蛋白-1α、巨噬细胞炎性蛋白-1β、血小板衍生生长因子-BB、调节激活正常T细胞表达和分泌因子、肿瘤坏死因子-α和血管内皮生长因子）的表达。
在检测的27种细胞因子中，与对照组相比，CFS/ME患者中只有IL-10显著降低。
这项初步调查表明，CFS/ME患者脑脊液中炎性细胞因子的紊乱可能导致CFS/ME中观察到的神经功能差异。
