Zhang Xiuting, Cai Yongjiang, Wang Lijing, Liu Hongchun, Wang Xiulin
First affiliated hospital of Zhengzhou University Department of Medicine, Zhengzhou, Henan Province, China.
Afr Health Sci. 2015 Mar;15(1):101-6. doi: 10.4314/ahs.v15i1.14.
Rhizoma Pinelliae is the dried tuber of Pinellia ternata (Thunb.) Breit. Modern pharmacological studies have shown that Rhizoma Pinelliae has antitussive, antiemetic, glandular secretion inhibiting and antitumor effects.
To optimize the processing technology of Rhizoma Pinelliae Praeparatum, and to study its anti-tumor effect.
Orthogonal design method was applied to analyze the effects of factors such as licorice concentration volume, soaking time and processing temperature on processing technology of Rhizoma Pinelliae Praeparatum; MTT assay and flow cytometry were used to determine the inhibitory effect of Rhizoma Pinelliae Praeparatum on Bel-7402 cells.
During the processing of Rhizoma Pinelliae Praeparatum, the size of influence of licorice concentration volume, soaking time and processing temperature on processing results of Rhizoma Pinelliae was: B>C>A in descending order, i.e. soaking time>processing temperature>licorice concentration volume, different concentrations of Rhizoma Pinelliae Praeparatum ethanol extracts could all exert inhibitory effect on the growth and proliferation of Bel-7402 cells, and with the increase of drug concentration and the extension of culture time, the cell proliferation inhibitory effect of Rhizoma Pinelliae Praeparatum ethanol extract became more and more evident. Apoptotic rate of 1.5 mg/ml Rhizoma Pinelliae Praeparatum ethanol extract group reached 13.53%, the difference was extremely significant compared with the control group. In conclusion the factor most influential to the processing technology of Rhizoma Pinelliae Praeparatum was soaking time, followed by processing temperature, the factor least influential was licorice concentration volume.
Rhizoma Pinelliae Praeparatum has inhibitory effect on growth and proliferation of Bel-7402 cells.
半夏为天南星科植物半夏Pinellia ternata (Thunb.) Breit.的干燥块茎。现代药理研究表明，半夏具有止咳、止吐、抑制腺体分泌及抗肿瘤等作用。
优化法半夏炮制工艺，并研究其抗肿瘤作用。
采用正交设计法，分析甘草浓度体积、浸泡时间、炮制温度等因素对法半夏炮制工艺的影响；采用MTT法和流式细胞术检测法半夏对Bel-7402细胞的抑制作用。
法半夏炮制过程中，甘草浓度体积、浸泡时间、炮制温度对法半夏炮制结果的影响大小依次为B>C>A，即浸泡时间>炮制温度>甘草浓度体积；不同浓度的法半夏乙醇提取物均能对Bel-7402细胞的生长和增殖产生抑制作用，且随着药物浓度的增加和培养时间的延长，法半夏乙醇提取物的细胞增殖抑制作用越来越明显。1.5mg/ml法半夏乙醇提取物组细胞凋亡率达13.53%，与对照组相比差异极显著。综上，对法半夏炮制工艺影响最大的因素为浸泡时间，其次为炮制温度，影响最小的因素为甘草浓度体积。
法半夏对Bel-7402细胞的生长和增殖具有抑制作用。
