Shahi Shahriar, Ghasemi Negin, Rahimi Saeed, Yavari Hamid Reza, Samiei Mohammad, Janani Maryam, Bahari Mahmood
Dental and Periodontal Research Center, Dental Faculty, Tabriz University of Medical Sciences, Tabriz, Iran;
Department of Endodontics, Dental Faculty, Tabriz University of Medical Sciences, Tabriz, Iran;
Iran Endod J. 2015;10(2):140-3. Epub 2015 Mar 18.
The aim of this study was to evaluate the effect of different mixing techniques on the pH and solubility of mineral trioxide aggregate (MTA) and calcium-enriched mixture (CEM).
Five samples were prepared from each biomaterial with different mixing techniques including hand-, amalgamator- or ultrasonic-mixing and were then placed in pre-weighted plastic tubes to determine their pH values. Each tube was then incubated in 10 mL deionized distilled water for 1 h at 37(º)C. An electrode was placed in the fluid in each flask at 24(º)C and the pH was recorded. In the next stage, six samples from each mixing technique/material were separately placed in glass bottles containing 50 mL of distilled water at 37(º)C for 1 h and were let dry for 1 h at 37(º)C. The samples' weights were measured and recorded twice. The procedure was repeated at 1-, 7- and 21-day intervals. Data were analyzed with the repeated measures ANOVA (for solubility) and two-way ANOVA (for pH) and then the post-hoc Tukey's test was done.
The pH of the materials was not significantly affected by mixing methods. (P=0.8 for CEM and P=0.1 for MTA). The solubility of all test groups was within the acceptable range (=3%). However, the solubility of CEM at 1- and 21-day intervals was significantly different (P=0.03 for 1 day and P=0.001 for 21 days). Different mixing techniques had significant effects on the solubility of MTA at the three time points (P=0.004, 0.003 and 0.002 for 1-, 7- and 21-day intervals, respectively).
The pH of biomaterials was not influenced by the mixing technique and their solubility was within the acceptable range.
本研究旨在评估不同混合技术对三氧化物矿物聚合体（MTA）和富钙混合物（CEM）的pH值及溶解性的影响。
采用不同混合技术（包括手动、研磨机或超声混合）从每种生物材料制备五个样本，然后将其置于预先称重的塑料管中以测定其pH值。接着，将每个管在37℃下于10 mL去离子蒸馏水中孵育1小时。在24℃下将电极置于每个烧瓶中的液体中并记录pH值。在下一阶段，将每种混合技术/材料的六个样本分别置于37℃下含有50 mL蒸馏水的玻璃瓶中1小时，并在37℃下干燥1小时。对样本重量进行两次测量和记录。该程序在1天、7天和21天的间隔重复进行。数据采用重复测量方差分析（用于溶解性）和双向方差分析（用于pH值）进行分析，然后进行事后Tukey检验。
材料的pH值不受混合方法的显著影响（CEM的P = 0.8，MTA的P = 0.1）。所有测试组的溶解性均在可接受范围内（≤3%）。然而，CEM在1天和21天间隔时的溶解性存在显著差异（1天的P = 0.03，21天的P = 0.001）。不同混合技术在三个时间点对MTA的溶解性有显著影响（1天、7天和21天间隔时的P分别为0.004、0.003和0.002）。
生物材料的pH值不受混合技术影响，其溶解性在可接受范围内。
