Shahi Shahriar, Bashirzadeh Asmar, Yavari Hamid Reza, Jafari Farnaz, Salem Milani Amin, Ghasemi Negin, Samiei Mohammad
Dental and Periodontal Research Center, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
J Dent Res Dent Clin Dent Prospects. 2017 Spring;11(2):84-89. doi: 10.15171/joddd.2017.016. Epub 2017 Jun 21.
of this study was to investigate the effect of different mixing methods (ultrasonic, amalgamator, and conventional) on the bacterial microleakage of white Portland cement (WPC) and white MTA (Tooth-colored Formula, Dentsply, Tulsa Dental, Tulsa, OK). A hundred human single-rooted permanent teeth were decoronated to obtain 14 mm of root length in all the samples. The root canals were cleaned, shaped and obturated. Three millimeters of each root apex were cut off and randomly divided into 6 groups of 15 each (3 groups for WMTA and 3 groups for WPC, each with 3 different mixing methods) and 2 positive and negative control groups (each containing 5 samples). Brain-heart infusion agar (BHI) suspension containing the bacterial species (ATCC 29212) was used for leakage assessment. Statistical analysis was carried out using descriptive statistics and Kaplan Mayer survival analysis with censored data and log rank test using SPSS 18. Statistical significance was set at P<0.05. The survival means in PC for conventional method, amalgamator, and ultrasonic were 80.2±13.64, 78.5±13.46 and 84.667±11.42 days, with 49.13±12.96, 66±13.32 and 69.07±11.5 days for MTA, respectively. The log rank test showed no significant differences between the three methods in each material (P>0.05). Bacterial microleakage in the studied samples was not significantly different in terms of the type of the mixing method.
本研究旨在调查不同混合方法（超声、研磨机和传统方法）对白色波特兰水泥（WPC）和白色矿物三氧化物凝聚体（牙色配方，登士柏，塔尔萨牙科，俄克拉何马州塔尔萨）细菌微渗漏的影响。100颗人类单根恒牙被截冠，以使所有样本的牙根长度达到14毫米。根管进行清理、预备和充填。将每个根尖3毫米截断，并随机分为6组，每组15个样本（WMTA组3组，WPC组3组，每组有3种不同混合方法）以及2个阳性和阴性对照组（每组含5个样本）。含有细菌菌株（ATCC 29212）的脑心浸液琼脂（BHI）悬液用于渗漏评估。使用描述性统计、带有删失数据的Kaplan Mayer生存分析以及SPSS 18进行对数秩检验进行统计分析。设定统计学显著性为P<0.05。传统方法、研磨机和超声法在PC中的生存均值分别为80.2±13.64、78.5±13.46和84.667±11.42天，MTA的分别为49.13±12.96、66±13.32和69.07±11.5天。对数秩检验显示每种材料的三种方法之间无显著差异（P>0.05）。在所研究样本中，细菌微渗漏在混合方法类型方面无显著差异。
