Heybar Habib, Alavi Seyed Mohammad, Farashahi Nejad Mehdi, Latifi Mahmood
Cardiology Department, Medical College, Golestan Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
Health Research Institute, Jundishapur Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
Jundishapur J Microbiol. 2015 Mar 21;8(3):e15476. doi: 10.5812/jjm.15476. eCollection 2015 Mar.
Although there is enough evidence that infectious agents such as Chlamydia pneumonia and Helicobacter pylori may play a pathogenic role in atherosclerosis, this role for cytomegalovirus (CMV) is yet controversial.
The aim of the present study was to detect CMV-DNA in atherosclerotic plaques in patients who underwent coronary artery bypass graft (CABG).
In this case-control study, candidates for CABG (cases) and patients with valvular or congenital malformation but without atherosclerotic plaques (controls) were studied from 2012 to 2013 at Golestan hospital, Ahvaz, IR Iran. Demographic and laboratory data were collected. Atherosclerotic and histological samples were obtained from visible plaques and from aorta by the surgeon. All the samples were examined for the presence of CMV-DNA by polymerase chain reaction (PCR) method using a commercial kit (SinaClon, Tehran, IR Iran).
The mean ages in case and control groups were 60.8 ± 6.8 and 57.5 ± 11.5 years, respectively, with no significant difference (P = 0.09). Thirty patients (54.5%) in case and 32 (58.2%) in control groups were male with no significant difference (P = 0.7). CMV-DNA was present in 8 (14.5%) of the cases and 2 (4%) of the controls. CMV-DNA was associated with higher risk of atherosclerosis (OR: 7.7, 95% CI = 1.1-51.4, P = 0.03). Of the total normal aortic samples (55 in cases and 55 in controls), there was no individual with simultaneous positive CMV-DNA among aortic atherosclerotic and normal tissue samples.
The presence of CMV-DNA in aortic plaques is associated with increased risk of atherosclerosis. CMV infection may be considered as an independent risk factor for this event.
尽管有充分证据表明肺炎衣原体和幽门螺杆菌等感染因子可能在动脉粥样硬化中发挥致病作用，但巨细胞病毒（CMV）在其中的作用仍存在争议。
本研究旨在检测接受冠状动脉搭桥术（CABG）患者动脉粥样硬化斑块中的CMV-DNA。
在这项病例对照研究中，2012年至2013年期间在伊朗阿瓦士的戈勒斯坦医院对CABG候选患者（病例组）和患有瓣膜或先天性畸形但无动脉粥样硬化斑块的患者（对照组）进行了研究。收集了人口统计学和实验室数据。外科医生从可见斑块和主动脉获取动脉粥样硬化及组织学样本。使用商业试剂盒（伊朗德黑兰的SinaClon）通过聚合酶链反应（PCR）方法检测所有样本中CMV-DNA的存在情况。
病例组和对照组的平均年龄分别为60.8±6.8岁和57.5±11.5岁，无显著差异（P = 0.09）。病例组30例患者（54.5%）和对照组32例患者（58.2%）为男性，无显著差异（P = 0.7）。病例组中有8例（14.5%）检测到CMV-DNA，对照组中有2例（4%）检测到CMV-DNA。CMV-DNA与动脉粥样硬化风险升高相关（比值比：7.7，95%置信区间=1.1 - 51.4，P = 0.03）。在所有正常主动脉样本（病例组55例，对照组55例）中，主动脉动脉粥样硬化和正常组织样本中均无同时CMV-DNA呈阳性的个体。
主动脉斑块中CMV-DNA的存在与动脉粥样硬化风险增加相关。CMV感染可被视为该事件的独立危险因素。
