Liu Qi-Di, Qin Kun-Ming, Shen Bao-Jia, Cai Hao, Cai Bao-Chang
Engineering Center of State Ministry of Education for Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China; Nanjing Haichang Chinese Medicine Group Co, Ltd, Nanjing 210061, China; Key Laboratory of State Administration of TCM for Standardization of Chinese Medicine Processing, Nanjing 210023, China.
Engineering Center of State Ministry of Education for Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Chin J Nat Med. 2015 Mar;13(3):222-31. doi: 10.1016/S1875-5364(15)30008-X.
The present study was designed to optimize the processing of Fructus Arctii by response surface methodology (RSM). Based on single factor studies, a three-variable, three-level Box-Behnken design (BBD) was used to monitor the effects of independent variables, including processing temperature and time, on the dependent variables. Response surfaces and contour plots of the contents of total lignans, chlorogenic acid, arctiin, and arctigenin were obtained through ultraviolet and visible (UV-Vis) monitoring and high performance liquid chromatography (HPLC). Fructus Arctii should be processed under heating in a pot at 311 °C, medicine at 119 °C for 123s with flipping frequently. The experimental values under the optimized processing technology were consistent with the predicted values. In conclusion, RSM is an effective method to optimize the processing of traditional Chinese medicine (TCM).
本研究旨在通过响应面法（RSM）优化牛蒡子的炮制工艺。基于单因素研究，采用三变量、三水平的Box-Behnken设计（BBD）来监测自变量（包括炮制温度和时间）对因变量的影响。通过紫外可见（UV-Vis）监测和高效液相色谱（HPLC）获得了总木脂素、绿原酸、牛蒡苷和牛蒡子苷元含量的响应面和等高线图。牛蒡子应在锅中于311℃加热，药材于119℃加热123秒并频繁翻动。优化炮制工艺下的实验值与预测值一致。总之，响应面法是优化中药炮制工艺的有效方法。
