牛蒡ITS序列与牛蒡子质量的相关性研究
[Study on correlation between ITS sequence of Arctium lappa and quality of Fructus Arctii].
作者信息
Xu Liang, Dou Deqiang, Wang Bing, Yang Yanyun, Kang Tingguo
机构信息
Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2011 Jul;36(14):1931-5.
OBJECTIVE
To study the correlation between ITS sequence of Arctium lappa and Fructus Arctii quality of different origin.
METHOD
The samples of Fructu arctii materials were collected from 26 different producing areas. Their ITS sequence were determined after polymerase chain reaction (PCR) and quality were evaluated through the determination of arctiin content by HPLC. Genetic diversity, genotype and correlation were analyzed by ClustalX (1.81), Mage 4.0, SPSS 13.0 statistical software.
RESULT
ITS sequence of A. was obtained from 26 samples, and was registered in the GenBank. Corresponding arctiin content of Fructus arctii and 1000-grain weight were determined. A. lappa genotype correlated with Fructus arctii quality by statistical analysis.
CONCLUSION
The research provided a foundation for revealing the molecular mechanism of Fructus arctii geoherbs.
目的
研究牛蒡ITS序列与不同产地牛蒡子质量的相关性。
方法
采集26个不同产地的牛蒡子药材样本。经聚合酶链反应（PCR）后测定其ITS序列，并通过高效液相色谱法测定牛蒡苷含量来评价质量。利用ClustalX（1.81）、Mage 4.0、SPSS 13.0统计软件分析遗传多样性、基因型及相关性。
结果
从26个样本中获得牛蒡的ITS序列，并在GenBank中注册。测定了相应牛蒡子的牛蒡苷含量及千粒重。经统计分析，牛蒡基因型与牛蒡子质量相关。
结论
本研究为揭示牛蒡道地药材的分子机制奠定了基础。
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