用于同时对多达25个焦平面进行三维偏振成像的多焦点偏振显微镜（MF-PolScope）。
MultiFocus Polarization Microscope (MF-PolScope) for 3D polarization imaging of up to 25 focal planes simultaneously.
作者信息
Abrahamsson Sara, McQuilken Molly, Mehta Shalin B, Verma Amitabh, Larsch Johannes, Ilic Rob, Heintzmann Rainer, Bargmann Cornelia I, Gladfelter Amy S, Oldenbourg Rudolf
出版信息
Opt Express. 2015 Mar 23;23(6):7734-54. doi: 10.1364/OE.23.007734.
Abstract
We have developed an imaging system for 3D time-lapse polarization microscopy of living biological samples. Polarization imaging reveals the position, alignment and orientation of submicroscopic features in label-free as well as fluorescently labeled specimens. Optical anisotropies are calculated from a series of images where the sample is illuminated by light of different polarization states. Due to the number of images necessary to collect both multiple polarization states and multiple focal planes, 3D polarization imaging is most often prohibitively slow. Our MF-PolScope system employs multifocus optics to form an instantaneous 3D image of up to 25 simultaneous focal-planes. We describe this optical system and show examples of 3D multi-focus polarization imaging of biological samples, including a protein assembly study in budding yeast cells.
摘要
我们开发了一种用于对活生物样本进行三维延时偏振显微镜成像的系统。偏振成像可揭示未标记以及荧光标记样本中亚微观特征的位置、排列和取向。光学各向异性是根据一系列样本由不同偏振态光照射的图像计算得出的。由于收集多个偏振态和多个焦平面所需的图像数量众多，三维偏振成像通常速度极慢。我们的多功能偏振仪（MF-PolScope）系统采用多焦点光学器件来形成多达25个同时聚焦平面的瞬时三维图像。我们描述了这个光学系统，并展示了生物样本的三维多焦点偏振成像示例，包括对出芽酵母细胞中蛋白质组装的研究。
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