一种用于直接检测泌尿生殖系统标本中沙眼衣原体的放射性rRNA:cDNA杂交检测法的评估
Evaluation of a radioactive rRNA:cDNA-hybridisation assay for the direct detection of Chlamydia trachomatis in urogenital specimens.
作者信息
Näher H, Niebauer B, Hartmann M, Söltz-Szöts J, Petzoldt D
机构信息
Department of Dermatology and Venereology, Ruprecht-Karls-University, Heidelberg, Federal Republic of Germany.
出版信息
Genitourin Med. 1989 Oct;65(5):319-22. doi: 10.1136/sti.65.5.319.
A radioactive cDNA probe complementary to chlamydial ribosomal RNA was used to detect C trachomatis in urogenital specimens. Of 37 specimens positive with cell culture 31 were confirmed by the rRNA:cDNA hybridisation test, the sensitivity being 83.8%. The specificity of the hybridisation test was 94.4%, as 186 of 197 specimens that were negative by cell culture were also negative when assessed by the hybridisation method. Given a prevalence of 15.8% the predictive values for positive and negative results were 73.8% and 96.9%, respectively. In additional experiments the possible role of microorganisms added to the specimen collection medium was investigated. However, no indication for crosshybridisation was found; at high concentrations microorganisms interfered with the test procedure.
一种与衣原体核糖体RNA互补的放射性cDNA探针被用于检测泌尿生殖系统标本中的沙眼衣原体。在37份细胞培养呈阳性的标本中，有31份通过rRNA:cDNA杂交试验得到确认，敏感性为83.8%。杂交试验的特异性为94.4%，因为在197份细胞培养呈阴性的标本中，有186份经杂交方法评估也呈阴性。在患病率为15.8%的情况下，阳性和阴性结果的预测值分别为73.8%和96.9%。在另外的实验中，研究了添加到标本采集培养基中的微生物可能发挥的作用。然而，未发现交叉杂交的迹象；在高浓度时，微生物会干扰检测程序。
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