多次传代未能提高衣原体回收率。
Failure of multiple passages to increase chlamydial recovery.
作者信息
Schachter J, Martin D H
机构信息
Department of Laboratory Medicine, University of California, San Francisco 94143.
出版信息
J Clin Microbiol. 1987 Oct;25(10):1851-3. doi: 10.1128/jcm.25.10.1851-1853.1987.
Abstract
Because of a previous publication indicating that increasing the number of blind passages to five resulted in a marked increase in Chlamydia trachomatis isolation rates, we evaluated a multiple-blind-passage system in vials. Cervical specimens from 119 pregnant women and 82 specimens from other sources were tested for chlamydial infection. The recovery rate for C. trachomatis was not markedly increased after the first blind passage. Thus, we conclude that multiple blind passages are not necessary to maximize the C. trachomatis isolation rate in a vial system.
摘要
由于之前有一篇发表的文章指出，将盲传次数增加到五次会导致沙眼衣原体分离率显著提高，我们评估了小瓶中的多次盲传系统。对119名孕妇的宫颈标本和82份其他来源的标本进行了衣原体感染检测。第一次盲传后，沙眼衣原体的回收率没有显著提高。因此，我们得出结论，在小瓶系统中，为了使沙眼衣原体分离率最大化，多次盲传并非必要。
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本文引用的文献
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