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本文引用的文献

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Microbe social skill: the cell-to-cell communication between microorganisms.微生物社交技能:微生物之间的细胞间通讯。
Sci Bull (Beijing). 2017 Apr 15;62(7):516-524. doi: 10.1016/j.scib.2017.02.010. Epub 2017 Feb 24.
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Stochastically reduced communities-Microfluidic compartments as model and investigation tool for soil microorganism growth in structured spaces.随机简化群落——微流体隔室作为结构化空间中土壤微生物生长的模型和研究工具。
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Review of Microfluidic Photobioreactor Technology for Metabolic Engineering and Synthetic Biology of Cyanobacteria and Microalgae.用于蓝藻和微藻代谢工程与合成生物学的微流控光生物反应器技术综述
Micromachines (Basel). 2016 Oct 11;7(10):185. doi: 10.3390/mi7100185.
4
Build your own soil: exploring microfluidics to create microbial habitat structures.自行构建土壤:探索微流控技术以创建微生物栖息结构。
ISME J. 2018 Feb;12(2):312-319. doi: 10.1038/ismej.2017.184. Epub 2017 Nov 14.
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A microfluidics-based in situ chemotaxis assay to study the behaviour of aquatic microbial communities.基于微流控技术的原位趋化性分析用于研究水生微生物群落的行为。
Nat Microbiol. 2017 Oct;2(10):1344-1349. doi: 10.1038/s41564-017-0010-9. Epub 2017 Aug 28.
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Emerging Droplet Microfluidics.新兴液滴微流控技术。
Chem Rev. 2017 Jun 28;117(12):7964-8040. doi: 10.1021/acs.chemrev.6b00848. Epub 2017 May 24.
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Coupling between distant biofilms and emergence of nutrient time-sharing.远距离生物膜之间的耦合以及营养物质分时利用的出现。
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Flow environment and matrix structure interact to determine spatial competition in biofilms.流动环境和基质结构相互作用,以决定生物膜中的空间竞争。
Elife. 2017 Jan 13;6:e21855. doi: 10.7554/eLife.21855.
9
Microbial competition in porous environments can select against rapid biofilm growth.多孔环境中的微生物竞争可能会抑制生物膜的快速生长。
Proc Natl Acad Sci U S A. 2017 Jan 10;114(2):E161-E170. doi: 10.1073/pnas.1525228113. Epub 2016 Dec 22.
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Microfluidic Studies of Biofilm Formation in Dynamic Environments.动态环境中生物膜形成的微流控研究
J Bacteriol. 2016 Sep 9;198(19):2589-95. doi: 10.1128/JB.00118-16. Print 2016 Oct 1.

微流体技术在实验生态学中的应用:空间因素的重要性

Application of Microfluidics in Experimental Ecology: The Importance of Being Spatial.

作者信息

Nagy Krisztina, Ábrahám Ágnes, Keymer Juan E, Galajda Péter

机构信息

Biological Research Centre, Institute of Biophysics, Hungarian Academy of Sciences, Szeged, Hungary.

Doctoral School of Multidisciplinary Medical Science, University of Szeged, Szeged, Hungary.

出版信息

Front Microbiol. 2018 Mar 20;9:496. doi: 10.3389/fmicb.2018.00496. eCollection 2018.

DOI:10.3389/fmicb.2018.00496
PMID:29616009
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5870036/
Abstract

Microfluidics is an emerging technology that is used more and more in biology experiments. Its capabilities of creating precisely controlled conditions in cellular dimensions make it ideal to explore cell-cell and cell-environment interactions. Thus, a wide spectrum of problems in microbial ecology can be studied using engineered microbial habitats. Moreover, artificial microfluidic ecosystems can serve as model systems to test ecology theories and principles that apply on a higher level in the hierarchy of biological organization. In this mini review we aim to demonstrate the versatility of microfluidics and the diversity of its applications that help the advance of microbiology, and in more general, experimental ecology.

摘要

微流控技术是一项正在兴起的技术,在生物学实验中的应用越来越广泛。它能够在细胞尺度上创造精确可控的条件,这使其成为探索细胞间和细胞与环境相互作用的理想工具。因此,利用工程化的微生物栖息地可以研究微生物生态学中的一系列广泛问题。此外,人工微流控生态系统可以作为模型系统,用于检验适用于生物组织层次结构中更高层次的生态学理论和原理。在本综述中,我们旨在展示微流控技术的多功能性及其应用的多样性,这些应用有助于推动微生物学乃至更广泛的实验生态学的发展。