一种用于通过流体动力学固定活的裂殖酵母细胞以进行超分辨率成像的微流控装置。
A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging.
作者信息
Bell Laurence, Seshia Ashwin, Lando David, Laue Ernest, Palayret Matthieu, Lee Steven F, Klenerman David
机构信息
Cambridge Nanoscience Centre, 11 J J Thomson Avenue, Cambridge CB3 0FF, United Kingdom.
Department of Biochemistry, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom.
出版信息
Sens Actuators B Chem. 2014 Mar 1;192:36-41. doi: 10.1016/j.snb.2013.10.002.
We describe a microfluidic device designed specifically for the reversible immobilisation of (Fission Yeast) cells to facilitate live cell super-resolution microscopy. Photo-Activation Localisation Microscopy (PALM) is used to create detailed super-resolution images within living cells with a modal accuracy of >25 nm in the lateral dimensions. The novel flow design captures and holds cells in a well-defined array with minimal effect on the normal growth kinetics. Cells are held over several hours and can continue to grow and divide within the device during fluorescence imaging.
我们描述了一种专门设计用于可逆固定（裂殖酵母）细胞的微流控装置，以促进活细胞超分辨率显微镜检查。光激活定位显微镜（PALM）用于在活细胞内创建详细的超分辨率图像，横向尺寸的模态精度>25 nm。这种新颖的流动设计将细胞捕获并保持在定义明确的阵列中，对正常生长动力学的影响最小。细胞可被保持数小时，并且在荧光成像期间能够在装置内继续生长和分裂。
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