使用合成非放射性寡核苷酸检测血清中的乙型肝炎病毒DNA
Detection of hepatitis B virus DNA in serum using synthetic non-radioactive oligonucleotides.
作者信息
Manzin A, Pauri P, Bagnarelli P, Brecciaroli F, Varaldo P E, Colloca S, Clementi M
机构信息
Institute of Microbiology, University of Ancona, Italy.
出版信息
J Clin Pathol. 1989 Nov;42(11):1206-10. doi: 10.1136/jcp.42.11.1206.
A rapid and simplified technique for detecting hepatitis B virus (HBV) DNA by spot hybridisation in the sera of patients with different clinical forms of HBV infection was investigated using enzyme conjugated synthetic oligodeoxyribonucleotides as probes. These are able to hybridize to the S and C regions of the HBV L(-) DNA strand. When compared with a complete 32P-labelled HBV DNA probe, the synthetic oligonucleotides provided a sensitive and quick method for the routine survey of HBV infection. Moreover, the DNA extraction procedure used allowed the spot hybridisation technique to be applied and read easily and the results obtained within a few hours. It is concluded that synthetic cold probes can be used in hybridisation assays HBV DNA detection as part of current clinical laboratory procedures.
使用酶联合成寡脱氧核糖核苷酸作为探针，研究了一种通过斑点杂交快速检测不同临床形式乙肝病毒（HBV）感染患者血清中HBV DNA的简化技术。这些探针能够与HBV L（-）DNA链的S区和C区杂交。与完整的32P标记HBV DNA探针相比，合成寡核苷酸为HBV感染的常规检测提供了一种灵敏且快速的方法。此外，所采用的DNA提取程序使斑点杂交技术易于应用和读取，且能在数小时内获得结果。得出的结论是，合成冷探针可作为当前临床实验室程序的一部分用于HBV DNA检测的杂交分析。
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