Vardiman John P, Moodie Nicole, Siedlik Jacob A, Kudrna Rebecca A, Graham Zachary, Gallagher Philip
Applied Physiology Laboratory, University of Kansas, Lawrence;
Exercise and Sport Science Department, Rockhurst University, Kansas City, MO;
J Athl Train. 2015 Jun;50(6):612-20. doi: 10.4085/1062-6050-50.1.12. Epub 2015 Apr 6.
Various modalities have been used to pretreat skeletal muscle to attenuate inflammation.
To determine the effects of short-wave diathermy (SWD) preheating treatment on inflammation and stress markers after eccentric exercise.
Controlled laboratory study.
University laboratory setting.
Fifteen male (age = 22 ± 4.9 years, height = 179.75 ± 9.56 cm, mass = 82.22 ± 12.67 kg) college-aged students.
INTERVENTION(S): Seven participants were selected randomly to receive 40 minutes of SWD heat treatment (HT), and 8 participants served as the control (CON) group and rested without SWD. Both groups completed 7 sets of 10 repetitions of a high-intensity eccentric exercise protocol (EEP) at 120% of the 1-repetition maximum (1-RM) leg extension.
MAIN OUTCOME MEASURE(S): We biopsied muscles on days 1, 3 (24 hours post-EEP), and 4 (48 hours post-EEP) and collected blood samples on days 1, 2 (4 hours post-EEP), 3, and 4. We determined 1-RM on day 2 (24 hours post-SWD) and measured 1-RM on days 3 and 4. We analyzed the muscle samples for interleukin 6 (IL-6), tumor necrosis factor α, and heat shock protein 70 and the blood for serum creatine kinase.
We found a group × time interaction for intramuscular IL-6 levels after SWD (F2,26 = 7.13, P = .003). The IL-6 decreased in HT (F1,6 = 17.8, P = .006), whereas CON showed no change (P > .05). We found a group × time interaction for tumor necrosis factor α levels (F2,26 = 3.71, P = .04), which increased in CON (F2,14 = 7.16, P = .007), but saw no changes for HT (P > .05). No group × time interactions were noted for 1-RM, heat shock protein 70, or creatine kinase (P > .05).
The SWD preheating treatment provided a treatment effect for intramuscular inflammatory myokines induced through high-intensity eccentric exercise but did not affect other factors associated with intense exercise and inflammation.
已采用多种方式对骨骼肌进行预处理以减轻炎症。
确定短波透热疗法（SWD）预热治疗对离心运动后炎症和应激标志物的影响。
对照实验室研究。
大学实验室环境。
15名男性（年龄=22±4.9岁，身高=179.75±9.56厘米，体重=82.22±12.67千克）大学生。
随机选择7名参与者接受40分钟的SWD热疗（HT），8名参与者作为对照组（CON），不接受SWD休息。两组均以1次最大重复量（1-RM）腿部伸展的120%完成7组，每组10次的高强度离心运动方案（EEP）。
在第1天、第3天（EEP后24小时）和第4天（EEP后48小时）对肌肉进行活检，并在第1天、第2天（EEP后4小时）、第3天和第4天采集血样。在第2天（SWD后24小时）测定1-RM，并在第3天和第4天测量1-RM。分析肌肉样本中的白细胞介素6（IL-6）、肿瘤坏死因子α和热休克蛋白70，以及血液中的血清肌酸激酶。
我们发现SWD后肌肉内IL-6水平存在组×时间交互作用（F2,26 = 7.13，P = .003）。HT组中IL-6水平降低（F1,6 = 17.8，P = .006），而CON组无变化（P>.05）。我们发现肿瘤坏死因子α水平存在组×时间交互作用（F2,26 = 3.71，P = .04），CON组中该水平升高（F2,14 = 7.16，P = .007），但HT组无变化（P>.05）。1-RM、热休克蛋白70或肌酸激酶未观察到组×时间交互作用（P>.05）。
SWD预热治疗对高强度离心运动诱导的肌肉内炎性肌因子有治疗效果，但不影响与剧烈运动和炎症相关的其他因素。
