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体内Z盘肌节成熟需要收缩活动。

Contractile activity is required for Z-disc sarcomere maturation in vivo.

作者信息

Geach Timothy J, Hirst Elizabeth M A, Zimmerman Lyle B

机构信息

Division of Developmental Biology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom.

出版信息

Genesis. 2015 May;53(5):299-307. doi: 10.1002/dvg.22851. Epub 2015 May 12.

DOI:10.1002/dvg.22851
PMID:25845369
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4676352/
Abstract

Sarcomere structure underpins structural integrity, signaling, and force transmission in the muscle. In embryos of the frog Xenopus tropicalis, muscle contraction begins even while sarcomerogenesis is ongoing. To determine whether contractile activity plays a role in sarcomere formation in vivo, chemical tools were used to block acto-myosin contraction in embryos of the frog X. tropicalis, and Z-disc assembly was characterized in the paralyzed dicky ticker mutant. Confocal and ultrastructure analysis of paralyzed embryos showed delayed Z-disc formation and defects in thick filament organization. These results suggest a previously undescribed role for contractility in sarcomere maturation in vivo.

摘要

肌节结构是肌肉结构完整性、信号传导和力传递的基础。在热带爪蟾胚胎中，即使肌节形成仍在进行，肌肉收缩就已开始。为了确定收缩活动在体内肌节形成中是否起作用，人们使用化学工具来阻断热带爪蟾胚胎中的肌动球蛋白收缩，并对瘫痪的“矮胖跳动者”突变体中的Z盘组装进行了表征。对瘫痪胚胎的共聚焦和超微结构分析显示Z盘形成延迟以及粗肌丝组织存在缺陷。这些结果表明收缩性在体内肌节成熟中具有此前未被描述的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a434/4676352/aad9d17fc38b/dvg0053-0299-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a434/4676352/223a810eab53/dvg0053-0299-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a434/4676352/f658113df6b6/dvg0053-0299-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a434/4676352/16e3e4493b99/dvg0053-0299-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a434/4676352/aad9d17fc38b/dvg0053-0299-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a434/4676352/223a810eab53/dvg0053-0299-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a434/4676352/f658113df6b6/dvg0053-0299-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a434/4676352/16e3e4493b99/dvg0053-0299-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a434/4676352/aad9d17fc38b/dvg0053-0299-f4.jpg

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