用于检测人细小病毒B19免疫球蛋白M的合成肽酶联免疫吸附测定法的评估
Evaluation of a synthetic-peptide enzyme-linked immunosorbent assay for immunoglobulin M to human parvovirus B19.
作者信息
Fridell E, Cohen B J, Wahren B
机构信息
Department of Virology, Karolinska Institute, Stockholm, Sweden.
出版信息
J Clin Microbiol. 1991 Jul;29(7):1376-81. doi: 10.1128/jcm.29.7.1376-1381.1991.
Abstract
A synthetic peptide corresponding to a part of the virus protein 1-virus protein 2 overlapping region of human parvovirus B19 was used in an indirect enzyme-linked immunosorbent assay. Antibodies of the immunoglobulin (Ig) M class were measured in serum samples from patients with erythema infectiosum and controls. In comparison with an IgM assay using native B19 viral antigen, the peptide antigen assay was 92% sensitive and 87% specific. B19 IgM reactivities were seen in a limited number of children with other viral diseases. Specific IgM reactivities to short synthetic viral peptides have previously been reported only with Epstein-Barr virus. Since other sources of viral antigen are limited, the peptide antigen assay may be a useful alternative for the diagnosis of B19-associated disease in human beings.
摘要
一种对应于人类细小病毒B19病毒蛋白1-病毒蛋白2重叠区域一部分的合成肽被用于间接酶联免疫吸附测定。在传染性红斑患者和对照的血清样本中检测免疫球蛋白（Ig）M类抗体。与使用天然B19病毒抗原的IgM测定相比，肽抗原测定的敏感性为92%，特异性为87%。在少数患有其他病毒疾病的儿童中也观察到了B19 IgM反应性。以前仅报道过针对爱泼斯坦-巴尔病毒的短合成病毒肽的特异性IgM反应性。由于其他病毒抗原来源有限，肽抗原测定可能是诊断人类B19相关疾病的一种有用替代方法。
相似文献
1
Evaluation of a synthetic-peptide enzyme-linked immunosorbent assay for immunoglobulin M to human parvovirus B19.用于检测人细小病毒B19免疫球蛋白M的合成肽酶联免疫吸附测定法的评估
J Clin Microbiol. 1991 Jul;29(7):1376-81. doi: 10.1128/jcm.29.7.1376-1381.1991.
2
Enzyme-linked immunosorbent assay for IgG and IgM antibodies against human parvovirus B19: use of monoclonal antibodies and viral antigen propagated in vitro.检测抗人细小病毒B19 IgG和IgM抗体的酶联免疫吸附测定：单克隆抗体和体外增殖病毒抗原的应用
J Virol Methods. 1989 Nov;26(2):171-81. doi: 10.1016/0166-0934(89)90146-8.
3
Detection of antibodies and antigens of human parvovirus B19 by enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法检测人细小病毒B19的抗体和抗原。
J Clin Microbiol. 1986 Oct;24(4):522-6. doi: 10.1128/jcm.24.4.522-526.1986.
4
Diagnostic of parvovirus B19 infection.细小病毒B19感染的诊断。
Behring Inst Mitt. 1990 Aug(85):35-8.
5
Human parvovirus B19: ELISA and immunoblot assays.人细小病毒B19：酶联免疫吸附测定和免疫印迹分析
J Virol Methods. 1988 Jun;20(2):155-68. doi: 10.1016/0166-0934(88)90149-8.
6
Chronic parvovirus B19 infection induces the production of anti-virus antibodies with autoantigen binding properties.慢性细小病毒B19感染诱导产生具有自身抗原结合特性的抗病毒抗体。
Eur J Immunol. 1998 Mar;28(3):936-48. doi: 10.1002/(SICI)1521-4141(199803)28:03<936::AID-IMMU936>3.0.CO;2-X.
7
Evaluation of commercial enzyme linked immunosorbent assay for detection of B19 parvovirus IgM and IgG.用于检测B19细小病毒IgM和IgG的商用酶联免疫吸附测定法的评估
J Clin Pathol. 1991 Oct;44(10):831-4. doi: 10.1136/jcp.44.10.831.
8
The use of labeled fusion protein for detection of B19 parvovirus IgM antibodies by an immunocapture test.
J Virol Methods. 1991 Apr;32(1):21-30. doi: 10.1016/0166-0934(91)90181-x.
9
A cyclized peptide for studies of human parvovirus B19 infection.一种用于研究人细小病毒B19感染的环化肽。
J Immunol Methods. 1991 Apr 8;138(1):125-8. doi: 10.1016/0022-1759(91)90072-n.
10
New oligopeptide immunoglobulin G test for human parvovirus B19 antibodies.用于检测人细小病毒B19抗体的新型寡肽免疫球蛋白G检测法
J Clin Microbiol. 1991 Mar;29(3):431-5. doi: 10.1128/jcm.29.3.431-435.1991.
引用本文的文献
1
Development & standardization of an in-house IgM indirect ELISA for the detection of parvovirus B19 infections.建立并标准化检测微小病毒 B19 感染的内部 IgM 间接 ELISA
Indian J Med Res. 2017 Sep;146(3):381-385. doi: 10.4103/ijmr.IJMR_225_16.
2
Evaluation of antibodies against a rubella virus neutralizing domain for determination of immune status.评估针对风疹病毒中和域的抗体以确定免疫状态。
Clin Diagn Lab Immunol. 2000 Nov;7(6):964-6. doi: 10.1128/CDLI.7.6.964-966.2000.
3
Peptide-based OspC enzyme-linked immunosorbent assay for serodiagnosis of Lyme borreliosis.基于肽的OspC酶联免疫吸附测定法用于莱姆病的血清学诊断。
J Clin Microbiol. 1998 Dec;36(12):3474-9. doi: 10.1128/JCM.36.12.3474-3479.1998.
4
Epitope mapping of B-cell determinants on the 15-kilodalton lipoprotein of Treponema pallidum (Tpp15) with synthetic peptides.用合成肽对梅毒螺旋体15千道尔顿脂蛋白（Tpp15）上B细胞决定簇进行表位作图。
Infect Immun. 1996 Jul;64(7):2457-66. doi: 10.1128/iai.64.7.2457-2466.1996.
5
Does parvovirus B19 have a role in rheumatoid arthritis?细小病毒B19在类风湿性关节炎中起作用吗？
Ann Rheum Dis. 1994 Feb;53(2):106-11. doi: 10.1136/ard.53.2.106.
6
Evaluation of five commercial tests for detection of immunoglobulin M antibodies to human parvovirus B19.用于检测人细小病毒B19免疫球蛋白M抗体的五种商业检测方法的评估
J Clin Microbiol. 1995 May;33(5):1363-5. doi: 10.1128/jcm.33.5.1363-1365.1995.
7
Mapping of B-cell determinants in the nucleocapsid protein of Puumala virus: definition of epitopes specific for acute immunoglobulin G recognition in humans.普马拉病毒核衣壳蛋白中B细胞决定簇的定位：人类急性免疫球蛋白G识别的表位定义
Clin Diagn Lab Immunol. 1995 Jan;2(1):82-6. doi: 10.1128/cdli.2.1.82-86.1995.
本文引用的文献
1
Febrile illness due to a parvovirus.细小病毒引起的发热性疾病。
Br Med J. 1980 Jun 28;280(6231):1580. doi: 10.1136/bmj.280.6231.1580.
2
Diagnostic assays with monoclonal antibodies for the human serum parvovirus-like virus (SPLV).用于人类血清细小病毒样病毒（SPLV）的单克隆抗体诊断检测法。
J Hyg (Lond). 1983 Aug;91(1):113-30. doi: 10.1017/s0022172400060095.
3
Human parvovirus aplasia: case due to cross infection in a ward.人类细小病毒所致全血细胞减少症：病房内交叉感染病例
Br Med J (Clin Res Ed). 1984 Mar 3;288(6418):681. doi: 10.1136/bmj.288.6418.681.
4
Frequency and specificity of varicella zoster virus IgM response.水痘带状疱疹病毒IgM反应的频率和特异性
J Virol Methods. 1982 Nov;5(3-4):219-27. doi: 10.1016/0166-0934(82)90012-x.
5
An interchangeable ELISA for cytomegalovirus antigen and antibody.一种用于巨细胞病毒抗原和抗体的可互换酶联免疫吸附测定法。
J Virol Methods. 1981 Apr;2(5):301-12. doi: 10.1016/0166-0934(81)90029-x.
6
Transmission of serum parvovirus-like virus by clotting-factor concentrates.凝血因子浓缩物传播血清细小病毒样病毒
Lancet. 1983 Aug 27;2(8348):482-4. doi: 10.1016/s0140-6736(83)90512-3.
7
Outbreak of aplastic crises in sickle cell anaemia associated with parvovirus-like agent.与细小病毒样病原体相关的镰状细胞贫血再生障碍危象暴发。
Lancet. 1981 Sep 19;2(8247):595-7. doi: 10.1016/s0140-6736(81)92739-2.
8
An outbreak of erythema infectiosum associated with human parvovirus infection.一起与人类细小病毒感染相关的传染性红斑暴发。
J Hyg (Lond). 1984 Aug;93(1):85-93. doi: 10.1017/s0022172400060964.
9
Nucleotide sequence and genome organization of human parvovirus B19 isolated from the serum of a child during aplastic crisis.从再生障碍性贫血危象患儿血清中分离出的人细小病毒B19的核苷酸序列和基因组结构
J Virol. 1986 Jun;58(3):921-36. doi: 10.1128/JVI.58.3.921-936.1986.
10
Characterization of capsid and noncapsid proteins of B19 parvovirus propagated in human erythroid bone marrow cell cultures.在人类红系骨髓细胞培养物中增殖的B19细小病毒衣壳蛋白和非衣壳蛋白的特性分析
J Virol. 1987 Aug;61(8):2627-30. doi: 10.1128/JVI.61.8.2627-2630.1987.
Zotero 插件