Nakamura T, Mori M, Yoshida T, Murakami N, Kato T, Sugihara J, Saito K, Moriwaki H, Tomita E, Muto Y
Rinsho Byori. 1989 Aug;37(8):911-7.
A molar ratio of free branched-chain amino acids to tyrosine (BTR) was determined in the plasma of patients with liver diseases using a new enzymatic method. In addition, clinical significance of BTR was studied by comparing particularly with that of Fischer's ratio (a molar ratio of branched-chain amino acids to aromatic amino acids (tyrosine+phenylalanine], which was obtained by conventional HPLC (Amino acid autoanalyzer, Hitachi 835). Following results were obtained: 1) Enzymatically determined branched-chain amino acids and tyrosine showed significant correlations with respective results obtained by HPLC (r = 0.937, 0.972). 2) Significant correlation was also found between enzymatically determined BTR and Fischer's ratio obtained by HPLC. Changes of BTR in clinical courses were found to be in parallel with those of Fischer's ratio. 3) BTR as well as Fischer's ratio correlated significantly with ICG R15, KICG, prothrombin time (%) and serum albumin level. 4) BTRs in patients with decompensated liver cirrhosis or with fulminant hepatitis were significantly lower than those in patients with acute or chronic hepatitis. In conclusion, new enzymatic assay of branched-chain amino acids and tyrosine as described here is quite simple method, and is also considered to be very useful parameter of the clinical conditions of patients with liver diseases, particularly representing the severity of liver diseases and the protein nutritional status.
采用一种新的酶法测定了肝病患者血浆中游离支链氨基酸与酪氨酸的摩尔比（BTR）。此外，通过特别与费舍尔比值（支链氨基酸与芳香族氨基酸（酪氨酸+苯丙氨酸）的摩尔比，通过传统高效液相色谱法（氨基酸自动分析仪，日立835）获得）进行比较，研究了BTR的临床意义。得到以下结果：1）酶法测定的支链氨基酸和酪氨酸与高效液相色谱法得到的各自结果显著相关（r = 0.937，0.972）。2）酶法测定的BTR与高效液相色谱法得到的费舍尔比值之间也发现显著相关。临床过程中BTR的变化与费舍尔比值的变化平行。3）BTR以及费舍尔比值与吲哚菁绿R15、KICG、凝血酶原时间（%）和血清白蛋白水平显著相关。4）失代偿期肝硬化或暴发性肝炎患者的BTR显著低于急性或慢性肝炎患者。总之，本文所述的支链氨基酸和酪氨酸的新酶法测定是一种非常简单的方法，也被认为是肝病患者临床状况的非常有用的参数，尤其能代表肝病的严重程度和蛋白质营养状况。
