严寒糖单胞菌属，新属，一种新型的嗜冷酯酶和蛋白酶产生菌，从南极洲分离得到。
Frigoriflavimonas asaccharolytica gen. nov., sp. nov., a novel psychrophilic esterase and protease producing bacterium isolated from Antarctica.
机构信息
Laboratorio de Ecología Microbiana Medioambiental, Facultad de Química, Universidad de la República, Montevideo, Uruguay.
Laboratorio de Microbiología, Facultad de Ciencias, Unidad Asociada del Instituto de Química Biológica, Universidad de la República, Montevideo, Uruguay.
出版信息
Antonie Van Leeuwenhoek. 2021 Dec;114(12):1991-2002. doi: 10.1007/s10482-021-01656-x. Epub 2021 Sep 20.
The rod-shaped and Gram-stain-negative bacterial strain 16F, isolated from an air sample collected at King George Island, maritime Antarctica, was investigated to determine its taxonomic status. Strain 16F is strictly aerobic, catalase positive, oxidase positive and non-motile. Strain 16F hydrolyses casein, lecithin, Tween 20, 60 and 80, but not aesculin, gelatin and starch. Growth of strain 16F is observed at 0-20 °C (optimum 10 °C), pH 5.0-8.0 (optimum pH 6.0), and in the presence of 0-2.0% NaCl (optimum 0.5%). The predominant menaquinone is MK-6, and the major fatty acids comprise anteiso-C and iso-C. The major polar lipids are phosphatidylethanolamine, ornithine lipid OL2, unidentified phospholipid PL1 and the unidentified lipids L3 and L6 lacking functional groups. The DNA G + C content based on the draft genome sequence is 32.3 mol%. Sequence analysis of the 16S rRNA gene indicates the highest similarity to Kaistella palustris 3A10 (95.4%), Kaistella chaponensis Sa 1147-06 (95.2%), Kaistella antarctica AT1013 (95.1%), Kaistella carnis NCTC 13525 (95.1%) and below 95.0% to other species with validly published names. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences places strain 16F in a distinct branch, indicating a separate lineage within the family Weeksellaceae. Based on the data from our polyphasic approach, 16F represents a novel species of a new genus, for which the name Frigoriflavimonas asaccharolytica gen. nov, sp. nov. is proposed. The type strain is 16F (= CCM 8975 = CGMCC No.1.16844).
从南极洲国王乔治岛采集的空气样本中分离到一株棒状革兰氏阴性细菌菌株 16F，对其进行了分类学研究。菌株 16F 为严格需氧、过氧化氢酶阳性、氧化酶阳性、非运动性。菌株 16F 可水解酪蛋白、卵磷脂、吐温 20、60 和 80，但不能水解马栗树皮苷、明胶和淀粉。菌株 16F 在 0-20°C（最适温度 10°C）、pH5.0-8.0（最适 pH6.0）和 0-2.0%NaCl（最适 0.5%）的条件下生长。主要的menaquinone 是 MK-6，主要的脂肪酸是 anteiso-C 和 iso-C。主要的极性脂是磷脂酰乙醇胺、鸟氨酸脂 OL2、未鉴定的磷脂 PL1 和缺乏功能基团的未鉴定脂 L3 和 L6。基于基因组草图的 DNA G+C 含量为 32.3mol%。16S rRNA 基因序列分析表明，该菌株与 Kaistella palustris 3A10（95.4%）、Kaistella chaponensis Sa 1147-06（95.2%）、Kaistella antarctica AT1013（95.1%）、Kaistella carnis NCTC 13525（95.1%）的相似度最高，与其他具有有效命名的物种的相似度低于 95.0%。基于 16S rRNA 基因和全基因组序列的系统发育分析将菌株 16F 置于一个独特的分支中，表明其在 Weeksellaceae 家族中属于一个单独的谱系。根据多相分类学研究数据，16F 代表了一个新属的新种，建议将其命名为 Frigoriflavimonas asaccharolytica gen. nov., sp. nov. 。模式菌株为 16F（=CCM 8975=CGMCC No.1.16844）。
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