Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany,
Antonie Van Leeuwenhoek. 2014 Mar;105(3):491-500. doi: 10.1007/s10482-013-0101-4. Epub 2013 Dec 31.
Four yellow pigmented strains (91A-561(T), 91A-576, 91A-593(T), and JM-1085(T)) isolated from plant materials, showed 97.2-98.7 % 16S rRNA gene sequence similarities among each other and were studied in a polyphasic approach for their taxonomic allocation. Cells of all four isolates were rod-shaped and stained Gram-negative. Comparative 16S rRNA gene sequence analysis showed that the four bacteria had highest sequence similarities to Chryseobacterium formosense (97.2-98.7 %), Chryseobacterium gwangjuense (97.1-97.8 %), and Chryseobacterium defluvii (94.6-98.0 %). Sequence similarities to all other Chryseobacterium species were below 97.5 %. Fatty acid analysis of the four strains showed Chryseobacterium typical profiles consisting of major fatty acids C15:0 iso, C15:0 iso 2-OH/C16:1 ω7c, C17:1 iso ω9c, and C17:0 iso 3-OH, but showed also slight differences. DNA-DNA hybridizations with type strains of C. gwangjuense, C. formosense, and C. defluvii resulted in values below 70 %. Isolates 91A-561(T) and 91A-576 showed DNA-DNA hybridization values >80 % indicating that they belonged to the same species; but nucleic acid fingerprinting showed that the two isolates represent two different strains. DNA-DNA hybridization results and the differentiating biochemical and chemotaxonomic properties showed, that both strains 91A-561(T) and 91A-576 represent a novel species, for which the name Chryseobacterium geocarposphaerae sp. nov. (type strain 91A-561(T)=LMG 27811(T)=CCM 8488(T)) is proposed. Strains 91A-593(T) and JM-1085(T) represent two additional new species for which we propose the names Chyrseobacterium zeae sp. nov. (type strain JM-1085(T)=LMG 27809(T), =CCM 8491(T)) and Chryseobacterium arachidis sp. nov. (type strain 91A-593(T)=LMG 27813(T), =CCM 8489(T)), respectively.
从植物材料中分离出的 4 株黄色色素菌株（91A-561(T)、91A-576、91A-593(T)和 JM-1085(T)）彼此之间的 16S rRNA 基因序列相似性为 97.2-98.7%，并通过多相研究方法对其分类进行了研究。所有 4 株分离物的细胞均为杆状，革兰氏染色阴性。比较 16S rRNA 基因序列分析表明，这 4 种细菌与 Formosense 黄杆菌（Chryseobacterium formosense）（97.2-98.7%）、Gwangjuense 黄杆菌（Chryseobacterium gwangjuense）（97.1-97.8%）和 Defluvii 黄杆菌（Chryseobacterium defluvii）（94.6-98.0%）具有最高的序列相似性。与所有其他 Chryseobacterium 种的序列相似性均低于 97.5%。4 株菌的脂肪酸分析显示 Chryseobacterium 典型图谱，由主要脂肪酸 C15:0 iso、C15:0 iso 2-OH/C16:1 ω7c、C17:1 iso ω9c 和 C17:0 iso 3-OH 组成，但也略有差异。与 Gwangjuense、Formosense 和 Defluvii 型菌株的 DNA-DNA 杂交产生的数值低于 70%。菌株 91A-561(T)和 91A-576 的 DNA-DNA 杂交值>80%，表明它们属于同一物种；但核酸指纹图谱显示，这两个分离株代表两个不同的菌株。DNA-DNA 杂交结果以及区分的生化和化学分类特性表明，这两个菌株 91A-561(T)和 91A-576 代表一个新的物种，建议将其命名为 Chryseobacterium geocarposphaerae sp. nov.（模式菌株 91A-561(T)=LMG 27811(T)=CCM 8488(T)）。菌株 91A-593(T)和 JM-1085(T)代表另外两个新物种，我们建议将其分别命名为 Chryseobacterium zeae sp. nov.（模式菌株 JM-1085(T)=LMG 27809(T)=CCM 8491(T)）和 Chryseobacterium arachidis sp. nov.（模式菌株 91A-593(T)=LMG 27813(T)=CCM 8489(T)）。
