Department of Biology and Geology, CITE II-B, University of Almeria, Agrifood Campus of International Excellence CeiA3, 04120 Almeria, Spain.
Department of Biology and Geology, CITE II-B, University of Almeria, Agrifood Campus of International Excellence CeiA3, 04120 Almeria, Spain.
Bioresour Technol. 2015;187:305-313. doi: 10.1016/j.biortech.2015.03.124. Epub 2015 Apr 3.
A comprehensive characterization of the culturable mycobiota associated to all stages of lignocellulose-based composting was achieved. A total of 77 different isolates were detected, 69 of which were identified on the basis of the 5.8-ITS region sequencing. All the isolates were assigned to the phyla Ascomycota and Basidiomycota, with prevalence of the Sordariomycetes (19) and Eurotiomycetes (17) classes. Penicillium was the most represented genus (11 species), while the species Gibellulopsis nigrescens and Microascus brevicaulis were detected at all the composting stages and showed the highest relative abundances. Fungal diversity decreased as the process proceed, while similarity between fungal communities associated to different samples were maximal for those phases closely connected chronologically and showing similar biological activity degree. Thus, the structure of the lignocellulose-based composting mycobiota can be divided into two major stages corresponding to bio-oxidative phase and maturation phase together with the final product, with a transitional cooling stage joining both of them.
实现了对木质纤维素基堆肥所有阶段相关可培养真菌群的全面描述。共检测到 77 种不同的分离株，其中 69 种是基于 5.8-ITS 区测序鉴定的。所有分离株被分配到子囊菌门和担子菌门，其中有优势的是 Sordariomycetes（19 种）和 Eurotiomycetes（17 种）纲。青霉是最具代表性的属（11 种），而 Gibellulopsis nigrescens 和 Microascus brevicaulis 这两个种则在所有堆肥阶段都被检测到，并且具有最高的相对丰度。真菌多样性随着过程的进行而减少，而与不同样品相关的真菌群落之间的相似性在时间上紧密相连且具有相似的生物活性程度的阶段最大。因此，木质纤维素基堆肥真菌群的结构可以分为两个主要阶段，对应生物氧化阶段和成熟阶段以及连接两者的最终产物冷却过渡阶段。
