Romanos Georgios E, Delgado-Ruiz Rafael A, Gómez-Moreno Gerardo, López-López Patricia J, Mate Sanchez de Val Jose E, Calvo-Guirado Jose Luis
Department of Periodontology, Stony Brook University, School of Dental Medicine, Stony Brook, NY, USA.
Department of Prosthodontics and Digital Technology, Stony Brook University, School of Dental Medicine, Stony Brook, New York, USA.
Clin Oral Implants Res. 2018 Jun;29(6):612-619. doi: 10.1111/clr.12592. Epub 2015 Apr 12.
This experimental study was designed to analyze the effect of different compressive forces on the bone regeneration around a particulate bone graft material.
Eighty 6-mm-diameter defects were created in the calvaria of 20 New Zealand rabbits (4 defects per rabbit calvaria). All the defects were filled with particles of synthetic bone. Two standardized compressive forces were then applied, 4.1 g to half the defects (Test A) and 8.2 g to the other half (Test B), all for 1 min. The graft sites were allowed to heal for 6 weeks, after which the rabbits were euthanized. The calvarium vault of each animal was extracted, radiographed, and prepared for histomorphometric analysis. The percentage of defect fill, bone density, new bone formation, and residual bone graft material were recorded, and the results were subjected to statistical analysis.
Histological evaluation found that defect closure among the Test A (lower compression) group ranged from 38.34 (95% lower CI) to 55.8 (95% upper CI) (mean 47 ± 8.5%), while among the Test B group (higher compression), it ranged from 81.26 (95% lower CI) to 95.32 (mean 88 ± 7.3%). Significantly more closure was achieved for the Test B group (P < 0.05). Histomorphometric comparison of the two groups found significantly more new bone formation, higher bone density, and a higher percentage of defect fill in the defects subjected to the higher compression level (P < 0.05).
Increasing the compressive force applied to bone graft particulate used to fill small defects created in rabbit calvaria appears to be beneficial.
本实验研究旨在分析不同压缩力对颗粒状骨移植材料周围骨再生的影响。
在20只新西兰兔的颅骨上制造80个直径6毫米的缺损（每只兔颅骨4个缺损）。所有缺损均填充合成骨颗粒。然后对一半缺损施加4.1克的标准化压缩力（试验A），对另一半缺损施加8.2克的标准化压缩力（试验B），均持续1分钟。让移植部位愈合6周，之后对兔子实施安乐死。提取每只动物的颅顶骨，进行X线摄影，并准备进行组织形态计量学分析。记录缺损填充百分比、骨密度、新骨形成情况和剩余骨移植材料，对结果进行统计分析。
组织学评估发现，试验A（较低压缩力）组的缺损闭合率在38.34（95%下限置信区间）至55.8（95%上限置信区间）之间（平均47±8.5%），而试验B组（较高压缩力）的缺损闭合率在81.26（95%下限置信区间）至95.32（平均88±7.3%）之间。试验B组实现的闭合率显著更高（P<0.05）。两组的组织形态计量学比较发现，在承受较高压缩力水平的缺损中，新骨形成的新骨形成显著更多、骨密度更高且缺损填充百分比更高（P<0.05）。
增加施加于用于填充兔颅骨小缺损的骨移植颗粒上的压缩力似乎是有益的。
