Kim Byoung-Jun, Kim Jae-Myung, Kim Bo-Ram, Lee So-Young, Kim GaNa, Jang Yun-Ho, Ryoo Soyoon, Jeon Che-Ok, Jin Hyun-Mi, Jeong Joseph, Lee Seon Ho, Lim Ji-Hun, Kook Yoon-Hoh, Kim Bum-Joon
Department of Biomedical Sciences, Microbiology and Immunology, Cancer Research Institute, and Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
Animal and Plant Health Research Department, Animal and Plant Quarantine Agency, Anyang 430-757, Republic of Korea.
Int J Syst Evol Microbiol. 2015 Jul;65(7):2277-2285. doi: 10.1099/ijs.0.000255. Epub 2015 Apr 13.
From the whole blood of Korean native cattle, Hanwoo (Bos taurus coreanae), a previously undescribed, rapidly growing, scotochromogenic isolate of the genus Mycobacterium is reported. Its 16S rRNA gene sequence, and the sequences of three other genes (hsp65, recA and rpoB) were unique and phylogenetic analysis based on 16S rRNA gene sequence (1420 bp) placed the organism into the rapidly growing Mycobacterium group close to Mycobacterium smegmatis (98.5% sequence similarity). However, phylogenetic analyses based on three different gene sequences (hsp65, recA and rpoB) revealed its location to be distinct from the branch of rapidly growing species. Culture and biochemical characteristics were generally similar to those of Mycobacterium fortuitum. Unique matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS profiles of lipids, unique fatty acid profile, unique mycolic acids profiles and a low DNA-DNA relatedness to M. fortuitum (23.6%) and M. smegmatis (39.7%) strongly supported the taxonomic status of this strain as a representative of a novel species of rapidly growing mycobacteria named Mycobacterium anyangense. The type strain is strain QIA-38(T) ( = JCM 30275(T) = KCTC 29443(T)).
据报道，从韩国本土牛韩牛（Bos taurus coreanae）的全血中分离出一种此前未被描述的、生长迅速的暗产色分枝杆菌属菌株。其16S rRNA基因序列以及其他三个基因（hsp65、recA和rpoB）的序列均独特，基于16S rRNA基因序列（1420 bp）的系统发育分析将该菌归入与耻垢分枝杆菌相近的快速生长分枝杆菌组（序列相似性为98.5%）。然而，基于三种不同基因序列（hsp65、recA和rpoB）的系统发育分析显示其位置与快速生长菌的分支不同。培养和生化特征总体上与偶然分枝杆菌相似。独特的脂质基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱图、独特的脂肪酸谱、独特的分枝菌酸谱以及与偶然分枝杆菌（23.6%）和耻垢分枝杆菌（39.7%）较低的DNA-DNA相关性有力地支持了该菌株作为一种名为安阳分枝杆菌的快速生长分枝杆菌新物种代表的分类地位。模式菌株为QIA-38(T)（=JCM 30275(T)=KCTC 29443(T)）。
